Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/8867
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dc.contributor.authorAydın, Hakan Berk-
dc.contributor.authorCheema, Jamal Ahmed-
dc.contributor.authorArnmanath, Gopal-
dc.contributor.authorToklucu, Cihan-
dc.contributor.authorYücel, Müge-
dc.contributor.authorÖzenler, Sezer-
dc.contributor.authorYıldız, Ümit Hakan-
dc.date.accessioned2020-07-18T08:34:04Z-
dc.date.available2020-07-18T08:34:04Z-
dc.date.issued2020-
dc.identifier.issn0039-9140-
dc.identifier.issn1873-3573-
dc.identifier.urihttps://doi.org/10.1016/j.talanta.2019.120581-
dc.identifier.urihttps://hdl.handle.net/11147/8867-
dc.description.abstractConjugated polyelectrolytes (CPEs) have been widely used as reporters in colorimetric assays targeting nucleic acids. CPEs provide naked eye detection possibility by their superior optical properties however, as concentration of target analytes decrease, trace amounts of nucleic acid typically yield colorimetric responses that are not readily perceivable by naked eye. Herein, we report a pixelated analysis approach for correlating colorimetric responses of CPE with nucleic acid concentrations down to 1 nM, in plasma samples, utilizing a smart phone with an algorithm that can perform analytical testing and data processing. The detection strategy employed relies on conformational transitions between single stranded nucleic acid-cationic CPE duplexes and double stranded nucleic acid-CPE triplexes that yield distinct colorimetric responses for enabling naked eye detection of nucleic acids. Cationic poly[N,N,N-triethyl-3-((4-methylthiophen-3-yl)oxy)propan-1-aminium bromide] is utilized as the CPE reporter deposited on a polyvinylidene fluoride (PVDF) membrane for nucleic acid assay. A smart phone application is developed to capture and digitize the colorimetric response of the individual pixels of the digital images of CPE on the PVDF membrane, followed by an analysis using the algorithm. The proposed pixelated approach enables precise quantification of nucleic acid assay concentrations, thereby eliminating the margin of error involved in conventional methodologies adopted for interpretation of colorimetric responses, for instance, RGB analysis. The obtained results illustrate that a ubiquitous smart phone could be utilized for point of care colorimetric nucleic acids assays in complex matrices without requiring sophisticated software or instrumentation.en_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofTalantaen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectConjugated polyelectrolyteen_US
dc.subjectPaper-based sensoren_US
dc.subjectPixelated analysisen_US
dc.subjectNucleic acid assayen_US
dc.subjectPoint-of-careen_US
dc.subjectDiagnostic toolen_US
dc.titlePixelated colorimetric nucleic acid assayen_US
dc.typeArticleen_US
dc.institutionauthorAydın, Hakan Berk-
dc.institutionauthorÖzenler, Sezer-
dc.institutionauthorYıldız, Ümit Hakan-
dc.institutionauthorToklucu, Cihan-
dc.institutionauthorYücel, Müge-
dc.departmentIzmir Institute of Technology. Chemistryen_US
dc.departmentIzmir Institute of Technology. Bioengineeringen_US
dc.departmentIzmir Institute of Technology. Computer Engineering-
dc.identifier.volume209en_US
dc.identifier.wosWOS:000509632900094en_US
dc.identifier.scopus2-s2.0-85076903010en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1016/j.talanta.2019.120581-
dc.identifier.pmid31892020en_US
dc.relation.doi10.1016/j.talanta.2019.120581en_US
dc.coverage.doi10.1016/j.talanta.2019.120581en_US
dc.identifier.scopusqualityQ1-
item.languageiso639-1en-
item.openairetypeArticle-
item.cerifentitytypePublications-
item.grantfulltextopen-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextWith Fulltext-
crisitem.author.dept01. Izmir Institute of Technology-
crisitem.author.dept04.01. Department of Chemistry-
Appears in Collections:Bioengineering / Biyomühendislik
Chemistry / Kimya
Computer Engineering / Bilgisayar Mühendisliği
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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