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https://hdl.handle.net/11147/8848
Title: | Human Immunodeficiency Virus Type-1 Tat Protein Induces Secretory Leukocyte Protease Inhibitor Expression in African Green Monkey but Not Human Cells | Authors: | Özdemir, Selçuk Şengez, Burcu Arslanoğlu, Alper |
Keywords: | African green monkey HIV-1 tat Secretory leukocyte protease inhibitor Anti-HIV-1 activity |
Publisher: | Springer | Abstract: | African monkeys are resistant to HIV-1 infection due to intrinsic restriction mechanisms found in their cells. However, although they can be infected by monkey-adapted modified HIV-1 particles that are designed to overcome known restriction factors, virus numbers drop to undetectable levels in immunocompetent animals. These results indicate the possibility of the presence of yet unidentified factor(s) that restrict HIV-1 in old-world monkey (OWM) cells after integration of the viral genome into the host cell chromosome. In the light of these findings, we hypothesized that OWMs might have evolved resistance mechanism(s) against HIV-1 by switching specific gene(s) on in response to the synthesis of viral proteins in infected cells. In an attempt to mimic post-infection status, we expressed HIV-1 Tat gene in African green monkey cells and compared the whole proteome with normal cells and identified secretory leukocyte protease inhibitor (SLPI), a protein with known extracellular anti-HIV-1 activity, as an over-expressed protein in the presence of HIV-1 Tat protein by 2D-PAGE and mass spectrometry analysis. We also showed that overexpression of SLPI in the presence of HIV-1 Tat was specific to monkey cells. Our results also suggest that SLPI had a previously undiscovered intracellular anti-HIV activity in addition to its extracellular activity. | URI: | https://doi.org/10.1007/s11262-020-01731-x https://hdl.handle.net/11147/8848 |
ISSN: | 0920-8569 1572-994X |
Appears in Collections: | Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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s11262-020-01731-x.pdf | 1.16 MB | Adobe PDF | View/Open |
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