Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/6844
Title: Nanofibrous gelatine scaffolds integrated with nerve growth factor-loaded alginate microspheres for brain tissue engineering
Authors: Büyüköz, Melda
Erdal, Esra
Alsoy Altınkaya, Sacide
Büyüköz, Melda
Alsoy Altınkaya, Sacide
Izmir Institute of Technology. Chemical Engineering
Keywords: Alginate microspheres
Brain tissue engineering
Controlled delivery
Gelatine scaffold
Nanofibers
Nerve growth factor
Issue Date: Feb-2018
Publisher: John Wiley and Sons Inc.
Source: Büyüköz, M., Erdal, E., and Alsoy Altınkaya, S. (2018). Nanofibrous gelatine scaffolds integrated with nerve growth factor-loaded alginate microspheres for brain tissue engineering. Journal of Tissue Engineering and Regenerative Medicine, 12(2), e707-e719. doi:10.1002/term.2353
Abstract: Neural regeneration research is designed in part to develop strategies for therapy after nerve damage due to injury or disease. In this study, a new gelatine-based biomimetic scaffold was fabricated for brain tissue engineering applications. A technique combining thermally induced phase separation and porogen leaching was used to create interconnected macropores and nanofibrous structure. To promote tissue regeneration processes, the scaffolds were integrated with nerve growth factor (NGF)-loaded alginate microspheres. The results showed that nanofibrous matrix could only be obtained when gelatine concentration was at least 7.5% (w/v). The scaffold with a modulus value (1.2 kPa) similar to that of brain tissue (0.5–1 kPa) was obtained by optimizing the heat treatment time, macropore size and gelatine concentration. The encapsulation efficiencies of NGF into 0.1% and 1% alginate microspheres were 85% and 100%, respectively. The release rate of NGF from the microspheres was controlled by the alginate concentration and the poly(L-lysine) coating. The immobilization of the microspheres in the scaffold reduced burst release and significantly extended the release period. The nanofibrous architecture and controlled release of NGF from the microspheres induced neurite extension of PC12 cells, demonstrating that the released NGF was in an active form. The results suggest that the scaffolds prepared in this study may have potential applications in brain tissue engineering due to topologic and mechanical properties similar to brain tissue and pore structure suitable for cell growth and differentiation.
URI: https://doi.org/10.1002/term.2353
http://hdl.handle.net/11147/6844
ISSN: 1932-6254
1932-6254
1932-6254
Appears in Collections:Chemical Engineering / Kimya Mühendisliği
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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