Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/6844
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dc.contributor.authorBüyüköz, Melda-
dc.contributor.authorErdal, Esra-
dc.contributor.authorAlsoy Altınkaya, Sacide-
dc.date.accessioned2018-03-27T12:39:46Z-
dc.date.available2018-03-27T12:39:46Z-
dc.date.issued2018-02-
dc.identifier.citationBüyüköz, M., Erdal, E., and Alsoy Altınkaya, S. (2018). Nanofibrous gelatine scaffolds integrated with nerve growth factor-loaded alginate microspheres for brain tissue engineering. Journal of Tissue Engineering and Regenerative Medicine, 12(2), e707-e719. doi:10.1002/term.2353en_US
dc.identifier.issn1932-6254-
dc.identifier.issn1932-6254-
dc.identifier.issn1932-6254-
dc.identifier.urihttps://doi.org/10.1002/term.2353-
dc.identifier.urihttp://hdl.handle.net/11147/6844-
dc.description.abstractNeural regeneration research is designed in part to develop strategies for therapy after nerve damage due to injury or disease. In this study, a new gelatine-based biomimetic scaffold was fabricated for brain tissue engineering applications. A technique combining thermally induced phase separation and porogen leaching was used to create interconnected macropores and nanofibrous structure. To promote tissue regeneration processes, the scaffolds were integrated with nerve growth factor (NGF)-loaded alginate microspheres. The results showed that nanofibrous matrix could only be obtained when gelatine concentration was at least 7.5% (w/v). The scaffold with a modulus value (1.2 kPa) similar to that of brain tissue (0.5–1 kPa) was obtained by optimizing the heat treatment time, macropore size and gelatine concentration. The encapsulation efficiencies of NGF into 0.1% and 1% alginate microspheres were 85% and 100%, respectively. The release rate of NGF from the microspheres was controlled by the alginate concentration and the poly(L-lysine) coating. The immobilization of the microspheres in the scaffold reduced burst release and significantly extended the release period. The nanofibrous architecture and controlled release of NGF from the microspheres induced neurite extension of PC12 cells, demonstrating that the released NGF was in an active form. The results suggest that the scaffolds prepared in this study may have potential applications in brain tissue engineering due to topologic and mechanical properties similar to brain tissue and pore structure suitable for cell growth and differentiation.en_US
dc.description.sponsorshipScientific and Technical Research Council of Turkey (TUBITAK 112M568)en_US
dc.language.isoenen_US
dc.publisherJohn Wiley and Sons Inc.en_US
dc.relationinfo:eu-repo/grantAgreement/TUBITAK/MAG/112M568en_US
dc.relation.ispartofJournal of Tissue Engineering and Regenerative Medicineen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAlginate microspheresen_US
dc.subjectBrain tissue engineeringen_US
dc.subjectControlled deliveryen_US
dc.subjectGelatine scaffolden_US
dc.subjectNanofibersen_US
dc.subjectNerve growth factoren_US
dc.titleNanofibrous gelatine scaffolds integrated with nerve growth factor-loaded alginate microspheres for brain tissue engineeringen_US
dc.typeArticleen_US
dc.authoridTR2091en_US
dc.institutionauthorBüyüköz, Melda-
dc.institutionauthorAlsoy Altınkaya, Sacide-
dc.departmentİzmir Institute of Technology. Chemical Engineeringen_US
dc.identifier.volume12en_US
dc.identifier.issue2en_US
dc.identifier.startpagee707en_US
dc.identifier.endpagee719en_US
dc.identifier.wosWOS:000425184900007en_US
dc.identifier.scopus2-s2.0-85017401839en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1002/term.2353-
dc.identifier.pmid27863118en_US
dc.relation.doi10.1002/term.2353en_US
dc.coverage.doi10.1002/term.2353en_US
dc.identifier.wosqualityQ2-
dc.identifier.scopusqualityQ1-
item.openairetypeArticle-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextWith Fulltext-
item.languageiso639-1en-
item.cerifentitytypePublications-
item.grantfulltextopen-
crisitem.author.dept03.02. Department of Chemical Engineering-
crisitem.author.dept03.02. Department of Chemical Engineering-
Appears in Collections:Chemical Engineering / Kimya Mühendisliği
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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