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https://hdl.handle.net/11147/6523
Title: | mRNA decay analysis in Drosophila melanogaster: Drug-induced changes in glutathione S-transferase D21 mRNA stability | Authors: | Akgül, Bünyamin Tu, Chen-Pei D. |
Keywords: | Drosophila melanogaster Glutathione transferase Messenger RNA RNA stability Drug effect |
Publisher: | Academic Press Inc. | Source: | Akgül, B., and Tu, C.-P. D. (2008). mRNA decay analysis in Drosophila melanogaster: Drug-induced changes in glutathione S-transferase D21 mRNA stability. Methods in Enzymology, 448, 285-297. doi:10.1016/S0076-6879(08)02615-3 | Abstract: | We have established an in vivo system to investigate mechanisms by which pentobarbital (PB), a psychoactive drug with a sedative effect, changes the rate of decay of gstD21 mRNA (encoding a Drosophila glutathione S-transferase). Here we describe methods for the use of hsp70 promoter-based transgenes and transgenic lines to determine mRNA half-lives by RNase protection assays in Drosophila. We are able to identify and map putative decay intermediates by cRT-PCR and DNA sequencing of the resulting clones. Our results indicate that the 3′-UTR of gstD21 mRNA is responsive to PB by regulating mRNA decay and that the cis-acting element(s) responsible for the PB-mediated stabilization resides in a 59 nucleotide sequence in the 3′-UTR of the gstD21 mRNA (Akgül and Tu, 2007). | URI: | http://doi.org/10.1016/S0076-6879(08)02615-3 http://hdl.handle.net/11147/6523 |
ISSN: | 0076-6879 1557-7988 |
Appears in Collections: | Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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