Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/6523
Title: mRNA decay analysis in Drosophila melanogaster: Drug-induced changes in glutathione S-transferase D21 mRNA stability
Authors: Akgül, Bünyamin
Tu, Chen-Pei D.
Keywords: Drosophila melanogaster
Glutathione transferase
Messenger RNA
RNA stability
Drug effect
Issue Date: 2008
Publisher: Academic Press Inc.
Source: Akgül, B., and Tu, C.-P. D. (2008). mRNA decay analysis in Drosophila melanogaster: Drug-induced changes in glutathione S-transferase D21 mRNA stability. Methods in Enzymology, 448, 285-297. doi:10.1016/S0076-6879(08)02615-3
Abstract: We have established an in vivo system to investigate mechanisms by which pentobarbital (PB), a psychoactive drug with a sedative effect, changes the rate of decay of gstD21 mRNA (encoding a Drosophila glutathione S-transferase). Here we describe methods for the use of hsp70 promoter-based transgenes and transgenic lines to determine mRNA half-lives by RNase protection assays in Drosophila. We are able to identify and map putative decay intermediates by cRT-PCR and DNA sequencing of the resulting clones. Our results indicate that the 3′-UTR of gstD21 mRNA is responsive to PB by regulating mRNA decay and that the cis-acting element(s) responsible for the PB-mediated stabilization resides in a 59 nucleotide sequence in the 3′-UTR of the gstD21 mRNA (Akgül and Tu, 2007).
URI: http://doi.org/10.1016/S0076-6879(08)02615-3
http://hdl.handle.net/11147/6523
ISSN: 0076-6879
1557-7988
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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