Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/6523
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dc.contributor.authorAkgül, Bünyamin-
dc.contributor.authorTu, Chen-Pei D.-
dc.date.accessioned2017-11-30T13:53:43Z-
dc.date.available2017-11-30T13:53:43Z-
dc.date.issued2008-
dc.identifier.citationAkgül, B., and Tu, C.-P. D. (2008). mRNA decay analysis in Drosophila melanogaster: Drug-induced changes in glutathione S-transferase D21 mRNA stability. Methods in Enzymology, 448, 285-297. doi:10.1016/S0076-6879(08)02615-3en_US
dc.identifier.issn0076-6879-
dc.identifier.issn1557-7988-
dc.identifier.urihttp://doi.org/10.1016/S0076-6879(08)02615-3-
dc.identifier.urihttp://hdl.handle.net/11147/6523-
dc.description.abstractWe have established an in vivo system to investigate mechanisms by which pentobarbital (PB), a psychoactive drug with a sedative effect, changes the rate of decay of gstD21 mRNA (encoding a Drosophila glutathione S-transferase). Here we describe methods for the use of hsp70 promoter-based transgenes and transgenic lines to determine mRNA half-lives by RNase protection assays in Drosophila. We are able to identify and map putative decay intermediates by cRT-PCR and DNA sequencing of the resulting clones. Our results indicate that the 3′-UTR of gstD21 mRNA is responsive to PB by regulating mRNA decay and that the cis-acting element(s) responsible for the PB-mediated stabilization resides in a 59 nucleotide sequence in the 3′-UTR of the gstD21 mRNA (Akgül and Tu, 2007).en_US
dc.description.sponsorshipNational Institute of Environmental Health Sciences (NIEHS) (ES02678)en_US
dc.language.isoenen_US
dc.publisherAcademic Press Inc.en_US
dc.relation.ispartofMethods in Enzymologyen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectDrosophila melanogasteren_US
dc.subjectGlutathione transferaseen_US
dc.subjectMessenger RNAen_US
dc.subjectRNA stabilityen_US
dc.subjectDrug effecten_US
dc.titlemRNA decay analysis in Drosophila melanogaster: Drug-induced changes in glutathione S-transferase D21 mRNA stabilityen_US
dc.typeArticleen_US
dc.authorid0000-0001-9877-9689en_US
dc.institutionauthorAkgül, Bünyamin-
dc.departmentIzmir Institute of Technology. Molecular Biology and Geneticsen_US
dc.identifier.volume448en_US
dc.identifier.startpage285en_US
dc.identifier.endpage297en_US
dc.identifier.wosWOS:000262252300015en_US
dc.identifier.scopus2-s2.0-57749181136en_US
dc.relation.publicationcategoryDiğeren_US
dc.identifier.doi10.1016/S0076-6879(08)02615-3-
dc.identifier.pmid19111182en_US
dc.relation.doi10.1016/S0076-6879(08)02615-3en_US
dc.coverage.doi10.1016/S0076-6879(08)02615-3en_US
dc.identifier.scopusqualityQ2-
item.openairetypeArticle-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextopen-
item.fulltextWith Fulltext-
item.cerifentitytypePublications-
crisitem.author.dept04.03. Department of Molecular Biology and Genetics-
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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