Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/6083
Title: Effect of PEG grafting density and hydrodynamic volume on gold nanoparticle-cell interactions: an investigation on cell cycle, apoptosis, and DNA damage
Authors: Uz, Metin
Bulmuş, Volga
Alsoy Altınkaya, Sacide
Uz, Metin
Bulmuş, Volga
Alsoy Altınkaya, Sacide
Keywords: Polyethylene oxides
Cell death
Metal nanoparticles
Gold
Grafting densities
PEG concentration
DNA
Issue Date: 14-Jun-2016
Publisher: American Chemical Society
Source: Uz, M., Bulmuş, V., and Alsoy Altınkaya, S. (2016). Effect of PEG grafting density and hydrodynamic volume on gold nanoparticle-cell interactions: An investigation on cell cycle, apoptosis, and DNA damage. Langmuir, 32(23), 5997-6009. doi:10.1021/acs.langmuir.6b01289
Abstract: In this study, interactions of polyethylene glycol (PEG)-coated gold nanoparticles (AuNPs) with cells were investigated with particular focus on the relationship between the PEG layer properties (conformation, grafting density, and hydrodynamic volume) and cell cycle arrest, apoptosis, and DNA damage. Steric hindrance and PEG hydrodynamic volume controlled the protein adsorption, whereas the AuNP core size and PEG hydrodynamic volume were primary factors for cell uptake and viability. At all PEG grafting densities, the particles caused significant cell cycle arrest and DNA damage against CaCo2 and PC3 cells without apoptosis. However, at a particular PEG grafting density (∼0.65 chains/nm2), none of these severe damages were observed on 3T3 cells indicating discriminating behavior of the healthy (3T3) and cancer (PC3 and CaCo2) cells. It was concluded that the PEG grafting density and hydrodynamic volume, tuned with the PEG concentration and AuNP size, played an important role in particle-cell interactions.
URI: https://doi.org/10.1021/acs.langmuir.6b01289
http://hdl.handle.net/11147/6083
ISSN: 0743-7463
1520-5827
Appears in Collections:Bioengineering / Biyomühendislik
Chemical Engineering / Kimya Mühendisliği
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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