• English
    • Türkçe
  • English 
    • English
    • Türkçe
  • Login
View Item 
  •   DSpace Home
  • 1. Fen Fakültesi / Faculty of Science
  • Chemistry / Kimya
  • View Item
  •   DSpace Home
  • 1. Fen Fakültesi / Faculty of Science
  • Chemistry / Kimya
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.
Realtime Access Map

Polythiophene derivative on quartz resonators for miRNA capture and assay

Thumbnail
View/Open
Makale (1.278Mb)
Date
2015-12
Author
Palaniappan, Al.
Cheema, Jamal Ahmed
Rajwar, Deepa
Ammanath, Gopal
Xiaohu, Liu
Seng Koon, Lim
Yi, Wang
Yıldız, Ümit Hakan
Liedberg, Bo
Metadata
Show full item record
Abstract
A novel approach for miRNA assay using a cationic polythiophene derivative, poly[3-(3′-N,N,N-triethylamino-1′-propyloxy)-4-methyl-2,5-thiophene hydrobromide] (PT), immobilized on a quartz resonator is proposed. The cationic PT enables capturing of all RNA sequences in the sample matrix via electrostatic interactions, resulting in the formation of PT-RNA duplex structures on quartz resonators. Biotinylated peptide nucleic acid (b-PNA) sequences are subsequently utilized for the RNA assay, upon monitoring the PT-RNA-b-PNA triplex formation. Signal amplification is achieved by anchoring avidin coated nanoparticles to b-PNA in order to yield responses at clinically relevant concentration regimes. Unlike conventional nucleic acid assay methodologies that usually quantify a specific sequence of RNA, the proposed approach enables the assay of any RNA sequence in the sample matrix upon hybridization with a PNA sequence complementary to the RNA of interest. As an illustration, successful detection of mir21, (a miRNA sequence associated with lung cancer) is demonstrated with a limit of detection of 400 pM. Furthermore, precise quantification of mir21 in plasma samples is demonstrated without requiring PCR and sophisticated instrumentation.
URI
http://doi.org/10.1039/c5an01663k
http://hdl.handle.net/11147/5669
Collections
  • Chemistry / Kimya [275]
  • Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection [3276]
  • WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection [2953]


DSpace software copyright © 2002-2015  DuraSpace
Contact Us | Send Feedback
Theme by 
@mire NV
 

 



| IZTECH OS Policy |
DSpace@IYTE Guide |

DSpace@IZTECH

by OpenAIRE
Advanced Search

Browse

All of DSpaceCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsTypeDepartmentPublisherCategoryLanguageAccess TypeIZTECH AuthorThis CollectionBy Issue DateAuthorsTitlesSubjectsTypeDepartmentPublisherCategoryLanguageAccess TypeIZTECH Author

My Account

LoginRegister

Statistics

View Usage Statistics

DSpace software copyright © 2002-2015  DuraSpace
Contact Us | Send Feedback
Theme by 
@mire NV
 

 


|| IZTECH OS Policy || DSpace@IYTE Guide || Library || IYTE || OAI-PMH ||

IZTECH Library, Gülbahçe Kampüsü - 35430 - Urla, İzmir / TURKEY
If you find any errors in content, please contact: openaccess@iyte.edu.tr.

Creative Commons Lisansı
DSpace@IZTECH by IYTE Institutional repository is licensed under a Creative Commons Attribution-Gayriticari-NoDerivs 3.0 Unported License.

DSpace@IZTECH is member of: