Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/5669
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dc.contributor.authorPalaniappan, Al.-
dc.contributor.authorCheema, Jamal Ahmed-
dc.contributor.authorRajwar, Deepa-
dc.contributor.authorAmmanath, Gopal-
dc.contributor.authorXiaohu, Liu-
dc.contributor.authorSeng Koon, Lim-
dc.contributor.authorYi, Wang-
dc.contributor.authorYıldız, Ümit Hakan-
dc.contributor.authorLiedberg, Bo-
dc.date.accessioned2017-06-01T08:34:26Z-
dc.date.available2017-06-01T08:34:26Z-
dc.date.issued2015-12-
dc.identifier.citationPalaniappan, A., Cheema, J. A., Rajwar, D., Ammanath, G., Xiaohu, L., Seng Koon, L., Yi, W., Yıldız, Ü. H., and Liedberg, B. (2015). Polythiophene derivative on quartz resonators for miRNA capture and assay, Analyst, 140(23), 7912-7917. doi:10.1039/c5an01663ken_US
dc.identifier.issn0003-2654-
dc.identifier.issn1364-5528-
dc.identifier.urihttp://doi.org/10.1039/c5an01663k-
dc.identifier.urihttp://hdl.handle.net/11147/5669-
dc.description.abstractA novel approach for miRNA assay using a cationic polythiophene derivative, poly[3-(3′-N,N,N-triethylamino-1′-propyloxy)-4-methyl-2,5-thiophene hydrobromide] (PT), immobilized on a quartz resonator is proposed. The cationic PT enables capturing of all RNA sequences in the sample matrix via electrostatic interactions, resulting in the formation of PT-RNA duplex structures on quartz resonators. Biotinylated peptide nucleic acid (b-PNA) sequences are subsequently utilized for the RNA assay, upon monitoring the PT-RNA-b-PNA triplex formation. Signal amplification is achieved by anchoring avidin coated nanoparticles to b-PNA in order to yield responses at clinically relevant concentration regimes. Unlike conventional nucleic acid assay methodologies that usually quantify a specific sequence of RNA, the proposed approach enables the assay of any RNA sequence in the sample matrix upon hybridization with a PNA sequence complementary to the RNA of interest. As an illustration, successful detection of mir21, (a miRNA sequence associated with lung cancer) is demonstrated with a limit of detection of 400 pM. Furthermore, precise quantification of mir21 in plasma samples is demonstrated without requiring PCR and sophisticated instrumentation.en_US
dc.language.isoengen_US
dc.publisherRoyal Society of Chemistryen_US
dc.relation.ispartofAnalysten_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectMicroRNAsen_US
dc.subjectPolymersen_US
dc.subjectPolythiopheneen_US
dc.subjectThiophenesen_US
dc.subjectQuartz crystalen_US
dc.titlePolythiophene derivative on quartz resonators for miRNA capture and assayen_US
dc.typearticleen_US
dc.authoridTR147447en_US
dc.contributor.institutionauthorYıldız, Ümit Hakan-
dc.contributor.departmentIzmir Institute of Technology. Chemistryen_US
dc.identifier.volume140en_US
dc.identifier.issue23en_US
dc.identifier.startpage7912en_US
dc.identifier.endpage7917en_US
dc.identifier.wosWOS:000365189900010
dc.identifier.scopusSCOPUS:2-s2.0-84947080668
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1039/c5an01663k-
dc.relation.doi10.1039/c5an01663ken_US
dc.coverage.doi10.1039/c5an01663ken_US
item.openairetypearticle-
item.cerifentitytypePublications-
item.languageiso639-1en-
item.fulltextWith Fulltext-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextopen-
crisitem.author.deptDepartment of Chemistry-
Appears in Collections:Chemistry / Kimya
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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