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https://hdl.handle.net/11147/5644
Title: | Gene reporter assay to validate microRNA targets in drosophila S2 cells | Authors: | Akgül, Bünyamin Göktaş, Çağdaş |
Keywords: | Drosophila Luciferase Reporter assay Target validation MicroRNAs |
Issue Date: | 2014 | Publisher: | Humana Press | Source: | Akgül, B., and Göktaş, Ç. (2014). Gene reporter assay to validate microRNA targets in drosophila S2 cells. Methods in Molecular Biology, 1107, 233-242. doi:10.1007/978-1-62703-748-8_14 | Abstract: | Bioinformatics programs have helped tremendously in identifying the targets of microRNAs, which are small noncoding RNAs that regulate gene expression posttranscriptionally. However, the partial complementarity between miRNAs and their targets hinders the accuracy of target prediction, necessitating the use of experimental validation procedures. Here, we describe a gene reporter assay typically used in our lab to validate putative miRNA-mRNA interactions in Drosophila S2 cells. | URI: | http://hdl.handle.net/11147/5644 http://doi.org/10.1007/978-1-62703-748-8_14 |
ISSN: | 1940-6029 1064-3745 |
Appears in Collections: | Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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