Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/5644
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dc.contributor.authorAkgül, Bünyamin-
dc.contributor.authorGöktaş, Çağdaş-
dc.date.accessioned2017-05-30T11:08:31Z-
dc.date.available2017-05-30T11:08:31Z-
dc.date.issued2014-
dc.identifier.citationAkgül, B., and Göktaş, Ç. (2014). Gene reporter assay to validate microRNA targets in drosophila S2 cells. Methods in Molecular Biology, 1107, 233-242. doi:10.1007/978-1-62703-748-8_14en_US
dc.identifier.issn1940-6029-
dc.identifier.issn1064-3745-
dc.identifier.urihttp://hdl.handle.net/11147/5644-
dc.identifier.urihttp://doi.org/10.1007/978-1-62703-748-8_14
dc.description.abstractBioinformatics programs have helped tremendously in identifying the targets of microRNAs, which are small noncoding RNAs that regulate gene expression posttranscriptionally. However, the partial complementarity between miRNAs and their targets hinders the accuracy of target prediction, necessitating the use of experimental validation procedures. Here, we describe a gene reporter assay typically used in our lab to validate putative miRNA-mRNA interactions in Drosophila S2 cells.en_US
dc.description.sponsorshipScientific and Technical Research Council of Turkey (104T144 to BA)en_US
dc.language.isoenen_US
dc.publisherHumana Pressen_US
dc.relation.ispartofMethods in Molecular Biologyen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectDrosophilaen_US
dc.subjectLuciferaseen_US
dc.subjectReporter assayen_US
dc.subjectTarget validationen_US
dc.subjectMicroRNAsen_US
dc.titleGene reporter assay to validate microRNA targets in drosophila S2 cellsen_US
dc.typeArticleen_US
dc.authoridTR37475en_US
dc.institutionauthorAkgül, Bünyamin-
dc.institutionauthorGöktaş, Çağdaş-
dc.departmentIzmir Institute of Technology. Molecular Biology and Geneticsen_US
dc.identifier.volume1107en_US
dc.identifier.startpage233en_US
dc.identifier.endpage242en_US
dc.identifier.wosWOS:000329167800015en_US
dc.identifier.scopus2-s2.0-84934435925en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1007/978-1-62703-748-8_14-
dc.identifier.pmid24272441en_US
dc.relation.doi10.1007/978-1-62703-748-8_14en_US
dc.coverage.doi10.1007/978-1-62703-748-8_14en_US
dc.identifier.scopusqualityQ3-
item.cerifentitytypePublications-
item.openairetypeArticle-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.languageiso639-1en-
item.grantfulltextopen-
item.fulltextWith Fulltext-
crisitem.author.dept04.03. Department of Molecular Biology and Genetics-
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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