Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/5620
Full metadata record
DC FieldValueLanguage
dc.contributor.authorCeylan, Çağatay-
dc.contributor.authorCamgöz, Aylin-
dc.contributor.authorBaran, Yusuf-
dc.date.accessioned2017-05-29T06:54:03Z-
dc.date.available2017-05-29T06:54:03Z-
dc.date.issued2012-08-
dc.identifier.citationCeylan, Ç, Camgöz, A. and Baran, Y. (2012). Macromolecular changes in nilotinib resistant k562 cells; An in vitro study by fourier transform infrared spectroscopy. Technology in Cancer Research and Treatment, 11(4), 333-344. doi:10.7785/tcrt.2012.500281en_US
dc.identifier.issn1533-0346-
dc.identifier.issn1533-0338-
dc.identifier.urihttp://dx.doi.org/10.7785/tcrt.2012.500281-
dc.identifier.urihttp://hdl.handle.net/11147/5620-
dc.description.abstractNilotinib is a second generation tyrosine kinase inhibitor which is used in both first and second line treatment of chronic myeloid leukemia (CML). In the present work, the effects of nilotinib resistance on K562 cells were investigated at the molecular level using Fourier transform infrared (FT-IR) spectroscopy. Human K562 CML cells were exposed to step-wise increasing concentrations of nilotinib, and sub-clones of K562 cells resistant to 50 nM nilotinib were generated and referred to as K562/NIL-50 cells. Antiproliferative effects of nilotinib were determined by XTT cell proliferation assay. Changes in macromolecules in parental and resistant cells were studied by FT-IR spectroscopy. Nilotinib resistance caused significant changes which indicated increases in the level of glycogen and membrane/lipid order. The amount of unsaturated lipids increased in the nilotinib resistant cells indicating lipid peroxidation. The total amount of lipids did not change significantly but the relative proportion of cholesterol and triglycerides altered considerably. Moreover, the transcriptional status decreased but metabolic turn-over increased as revealed by the FT-IR spectra. In addition, changes in the proteome and structural changes in both proteins and the nucleus were observed in the K562/NIL-50 cells. Protein secondary structural analyses revealed that alpha helix structure and random coil structure decreased, however, anti-parallel beta sheet structure, beta sheet structure and turns structure increased. These results indicate that the FT-IR technique provides a method for analyzing drug resistance related structural changes in leukemia and other cancer types.en_US
dc.description.sponsorshipTUBITAK (107S317); Turkish Academy of Sciencesen_US
dc.language.isoenen_US
dc.publisherSAGE Publications Inc.en_US
dc.relationinfo:eu-repo/grantAgreement/TUBITAK/TBAG/SBAG/107S317en_US
dc.relation.ispartofTechnology in Cancer Research and Treatmenten_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectNilotiniben_US
dc.subjectTyrosine kinase inhibitor resistanceen_US
dc.subjectChronic myeloid leukemiaen_US
dc.subjectFourier transform infrared spectroscopyen_US
dc.subjectTriacylglycerolen_US
dc.titleMacromolecular changes in nilotinib resistant k562 cells; An in vitro study by fourier transform infrared spectroscopyen_US
dc.typeArticleen_US
dc.authoridTR45775en_US
dc.authoridTR119193en_US
dc.contributor.departmentIzmir Institute of Technology. Food Engineeringen_US
dc.identifier.volume11en_US
dc.identifier.issue4en_US
dc.identifier.startpage333en_US
dc.identifier.endpage344en_US
dc.identifier.wosWOS:000306499500004
dc.identifier.scopusSCOPUS:2-s2.0-84863913864
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.7785/tcrt.2012.500281-
dc.relation.doi10.7785/tcrt.2012.500281en_US
dc.coverage.doi10.7785/tcrt.2012.500281en_US
item.cerifentitytypePublications-
item.grantfulltextopen-
item.fulltextWith Fulltext-
item.openairetypeArticle-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
crisitem.author.deptDepartment of Food Engineering-
crisitem.author.deptDepartment of Molecular Biology and Genetics-
Appears in Collections:Food Engineering / Gıda Mühendisliği
Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
Files in This Item:
File Description SizeFormat 
5620.pdfMakale1.52 MBAdobe PDFThumbnail
View/Open
Show simple item record

CORE Recommender

SCOPUSTM   
Citations

9
checked on Sep 25, 2021

WEB OF SCIENCETM
Citations

11
checked on Sep 25, 2021

Page view(s)

40
checked on Sep 25, 2021

Download(s)

30
checked on Sep 25, 2021

Google ScholarTM

Check

Altmetric


Items in GCRIS Repository are protected by copyright, with all rights reserved, unless otherwise indicated.