Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/4730
Title: Identification of extracellular enzyme producing alkalophilic bacilli from Izmir province by 16S-ITS rDNA RFLP
Authors: Akbalık, Güney
Güneş, Hatice
Yavuz, Elif
Yaşa, İhsan
Harsa, Hayriye Şebnem
Elmacı, Zehra Seda
Yenidünya, Ali Fazıl
Akbalık, Güney
Güneş, Hatice
Yavuz, Elif
Harsa, Hayriye Şebnem
Elmacı, Zehra Seda
Yenidünya, Ali Fazıl
Keywords: 16S-ITS rDNA-RFLP
Extracellular enzyme screening
Similarity analysis
Posibacteria
Alkalophilic Bacillus
Issue Date: 2004
Publisher: John Wiley and Sons Inc.
Source: Akbalık, G., Güneş, H., Yavuz, E., Yaşa, İ., Harsa, H. Ş., Elmacı, Z. S., and Yenidünya, A. F. (2004). Identification of extracellular enzyme producing alkalophilic bacilli from Izmir province by 16S-ITS rDNA RFLP. Journal of Applied Microbiology, 97(4), 766-773. doi:10.1111/j.1365-2672.2004.02357.x
Abstract: Aims: To screen industrially important extracellular enzymes from the newly isolated alkalophilic bacilli and to characterize them by phenotypic and 16S-internal transcribed spacer (ITS) rDNA restriction pattern analysis. Methods and Results: Three different environmental samples, soil, leather and horse faeces, were collected within the province of Izmir. Isolates grown on Horikoshi-I medium for 24 h at 37°C were screened for extracellular enzyme activity by using eight different substrates: birchwood xylan, carboxymethylcellulose, casein, citrus pectin, polygalacturonic acid, soluble starch, and Tween 20 and 80. In total, 115 extracellular enzyme-producing bacilli were obtained. Casein was hydrolysed by 78%, soluble starch by 67%, citrus pectin by 63%, polygalacturonic acid by 62%, Tween 20 by 34%, birchwood xylan by 16%, Tween 80 by 12%, and carboxymethylcellulose by 3% of the isolates. The isolates were differentiated into 19 distinct homology groups by the 16S-ITS rDNA restriction pattern analysis. Conclusions: Eight different extracellular enzyme activities were determined in 115 endospore forming bacilli. The largest 16S-ITS rDNA homology group (HT1) included 36% of the isolates, 98% of which degraded casein, polygalacturonic acid, pectin and starch. Significance and Impact of the Study: This study is the first report on the characterization of the industrial enzyme-producing alkalophilic bacilli by 16S-ITS rDNA restriction fragment length polymorphism (RFLP). Restriction profiles of 64% of the isolates were found to be different from those of five reference strains used.
URI: http://doi.org/10.1111/j.1365-2672.2004.02357.x
http://hdl.handle.net/11147/4730
ISSN: 1364-5072
1365-2672
1364-5072
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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