Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/4730
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dc.contributor.authorAkbalık, Güney-
dc.contributor.authorGüneş, Hatice-
dc.contributor.authorYavuz, Elif-
dc.contributor.authorYaşa, İhsan-
dc.contributor.authorHarsa, Hayriye Şebnem-
dc.contributor.authorElmacı, Zehra Seda-
dc.contributor.authorYenidünya, Ali Fazıl-
dc.date.accessioned2016-06-07T07:33:20Z-
dc.date.available2016-06-07T07:33:20Z-
dc.date.issued2004-
dc.identifier.citationAkbalık, G., Güneş, H., Yavuz, E., Yaşa, İ., Harsa, H. Ş., Elmacı, Z. S., and Yenidünya, A. F. (2004). Identification of extracellular enzyme producing alkalophilic bacilli from Izmir province by 16S-ITS rDNA RFLP. Journal of Applied Microbiology, 97(4), 766-773. doi:10.1111/j.1365-2672.2004.02357.xen_US
dc.identifier.issn1364-5072-
dc.identifier.issn1365-2672-
dc.identifier.issn1364-5072-
dc.identifier.urihttp://doi.org/10.1111/j.1365-2672.2004.02357.x-
dc.identifier.urihttp://hdl.handle.net/11147/4730-
dc.description.abstractAims: To screen industrially important extracellular enzymes from the newly isolated alkalophilic bacilli and to characterize them by phenotypic and 16S-internal transcribed spacer (ITS) rDNA restriction pattern analysis. Methods and Results: Three different environmental samples, soil, leather and horse faeces, were collected within the province of Izmir. Isolates grown on Horikoshi-I medium for 24 h at 37°C were screened for extracellular enzyme activity by using eight different substrates: birchwood xylan, carboxymethylcellulose, casein, citrus pectin, polygalacturonic acid, soluble starch, and Tween 20 and 80. In total, 115 extracellular enzyme-producing bacilli were obtained. Casein was hydrolysed by 78%, soluble starch by 67%, citrus pectin by 63%, polygalacturonic acid by 62%, Tween 20 by 34%, birchwood xylan by 16%, Tween 80 by 12%, and carboxymethylcellulose by 3% of the isolates. The isolates were differentiated into 19 distinct homology groups by the 16S-ITS rDNA restriction pattern analysis. Conclusions: Eight different extracellular enzyme activities were determined in 115 endospore forming bacilli. The largest 16S-ITS rDNA homology group (HT1) included 36% of the isolates, 98% of which degraded casein, polygalacturonic acid, pectin and starch. Significance and Impact of the Study: This study is the first report on the characterization of the industrial enzyme-producing alkalophilic bacilli by 16S-ITS rDNA restriction fragment length polymorphism (RFLP). Restriction profiles of 64% of the isolates were found to be different from those of five reference strains used.en_US
dc.language.isoenen_US
dc.publisherJohn Wiley and Sons Inc.en_US
dc.relation.ispartofJournal of Applied Microbiologyen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subject16S-ITS rDNA-RFLPen_US
dc.subjectExtracellular enzyme screeningen_US
dc.subjectSimilarity analysisen_US
dc.subjectPosibacteriaen_US
dc.subjectAlkalophilic Bacillusen_US
dc.titleIdentification of extracellular enzyme producing alkalophilic bacilli from Izmir province by 16S-ITS rDNA RFLPen_US
dc.typeArticleen_US
dc.authoridTR2082en_US
dc.authoridTR36501en_US
dc.authoridTR9626en_US
dc.authoridTR42080en_US
dc.institutionauthorAkbalık, Güney-
dc.institutionauthorGüneş, Hatice-
dc.institutionauthorYavuz, Elif-
dc.institutionauthorHarsa, Hayriye Şebnem-
dc.institutionauthorElmacı, Zehra Seda-
dc.institutionauthorYenidünya, Ali Fazıl-
dc.departmentİzmir Institute of Technology. Molecular Biology and Geneticsen_US
dc.identifier.volume97en_US
dc.identifier.issue4en_US
dc.identifier.startpage766en_US
dc.identifier.endpage773en_US
dc.identifier.wosWOS:000223817600012en_US
dc.identifier.scopus2-s2.0-4944247722en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1111/j.1365-2672.2004.02357.x-
dc.identifier.pmid15357726en_US
dc.relation.doi10.1111/j.1365-2672.2004.02357.xen_US
dc.coverage.doi10.1111/j.1365-2672.2004.02357.xen_US
local.message.claim2022-06-03T16:38:19.997+0300|||rp02904|||submit_approve|||dc_contributor_author|||None*
local.message.claim2022-06-16T10:28:54.985+0300|||rp03047|||submit_approve|||dc_contributor_author|||None*
dc.identifier.wosqualityQ2-
dc.identifier.scopusqualityQ1-
item.languageiso639-1en-
item.grantfulltextopen-
item.fulltextWith Fulltext-
item.cerifentitytypePublications-
item.openairetypeArticle-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
crisitem.author.dept04.03. Department of Molecular Biology and Genetics-
crisitem.author.dept01. Izmir Institute of Technology-
crisitem.author.dept03.08. Department of Food Engineering-
crisitem.author.dept04.03. Department of Molecular Biology and Genetics-
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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