Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/2430
Title: Development of practical HPLC methods for the separation and determination of eggplant steroidal glycoalkaloids and their aglycones
Authors: Eanes, Ritchie C.
Tek, Neslihan
Kırsoy, Öyküm
Frary, Anne
Doğanlar, Sami
Almeida, Adelia E.
Eanes, Ritchie C.
Tek, Neslihan
Kırsoy, Öyküm
Frary, Anne
Doğanlar, Sami
Izmir Institute of Technology. Chemistry
Izmir Institute of Technology. Molecular Biology and Genetics
Keywords: Organic compounds
Chaconine
Eggplant
Solamargine
Solanidine
Steroidal glycoalkaloids
Issue Date: Jan-2008
Publisher: Taylor and Francis Ltd.
Source: Eanes, R. C., Tek, N., Kırsoy, Ö., Frary, A., Doğanlar, S., and Almeida, A. E. (2008). Development of practical HPLC methods for the separation and determination of eggplant steroidal glycoalkaloids and their aglycones. Journal of Liquid Chromatography and Related Technologies, 31(7), 984-1000. doi:10.1080/10826070801924741
Abstract: A practical set of HPLC methods was developed for the separation and determination of the eggplant steroidal glycoalkaloids, solanine, chaconine, solasonine, solamargine, and their aglycones, solasodine and solanidine. A gradient method was initially developed, but proved to be neither robust nor practical. Three separate isocratic methods using acetonitrile and ammonium dihydrogen phosphate were developed and shown to be more repeatable, less subject to fluctuations in mobile phase composition, and less time consuming. The effect of adjusting buffer pH, column temperature, and buffer type (triethylammonium phosphate vs. ammonium dihydrogen phosphate) were evaluated. It was also discovered that, by addition of 10% methanol to the acetonitrile portion of the mobile phase, more control over the separations was possible. The use of methanol as a mobile phase entrainer greatly improved separations in some cases and its effectiveness was also dependent upon column temperature. Assessments of the method recovery, limit of detection, and limit of quantitation were made using extracts from S. melongena and S. linnaeanum.
URI: http://doi.org/10.1080/10826070801924741
http://hdl.handle.net/11147/2430
ISSN: 1082-6076
1082-6076
Appears in Collections:Chemistry / Kimya
Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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