Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/2277
Title: Regulation of mRNA stability through a pentobarbital-responsive element
Authors: Akgül, Bünyamin
Tu, Chen-Pei D.
Akgül, Bünyamin
Izmir Institute of Technology. Molecular Biology and Genetics
Keywords: Heat shock
mRNA decay
Pentobarbital
Polysome
Issue Date: Mar-2007
Publisher: Elsevier Ltd.
Source: Akgül, B., and Tu, C. P. D. (2007). Regulation of mRNA stability through a pentobarbital-responsive element. Archives of Biochemistry and Biophysics, 459(1), 143-150. doi:10.1016/j.abb.2006.10.026
Abstract: Pentobarbital, a general anesthetic and non-genotoxic carcinogen, can induce gene expression by activating transcription. In the Drosophila glutathione S-transferase D21 (gstD21) gene, pentobarbital's regulatory influence extends to the level of mRNA turnover. Transcribed from an intronless gene, gstD21 mRNA is intrinsically very labile. But exposure to pentobarbital renders it stabilized beyond what can be attributed to transcriptional activation. We aim here to identify cis-acting element(s) of gstD21 mRNA as contributors to the molecule's pentobarbital-mediated stabilization. In the context of hsp70 5′UTR and the 3′UTR of act5C, gstD21 mRNA, minus its native UTRs, is stable. Maintaining the same context of heterologous UTRs, we can reconstitute using the full-length gstD21 sequence the inherent instability of gstD21 mRNA and its stabilization by pentobarbital. Transgenic flies that express these chimeric gstD21 mRNA exhibit decay intermediates lacking 3′UTR, which are not stabilized by PB treatment. The 3′UTR sequence, when inserted downstream from a reporter transcript, stabilizes it 1.6-fold under PB treatment. The analysis of the decay intermediates suggests a polysome-associated decay pattern. We propose a regulatory model that features a 59-nucleotide pentobarbital-responsive element (PBRE) in the 3′UTR of gstD21 mRNA.
URI: http://doi.org/10.1016/j.abb.2006.10.026
http://hdl.handle.net/11147/2277
ISSN: 0003-9861
0003-9861
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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