Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/12390
Title: 3D printed gelatin/decellularized bone composite scaffolds for bone tissue engineering: Fabrication, characterization and cytocompatibility study
Authors: Kara, Aylin
Distler, Thomas
Polley, Christian
Schneidereit, Dominik
Seitz, Hermann
Friedrich, Oliver
Tıhmınlıoğlu, Funda
Boccaccini, Aldo R
01. Izmir Institute of Technology
Friedrich-Alexander Universität Erlangen-Nürnberg
Universität Rostock
Friedrich-Alexander Universität Erlangen-Nürnberg
Universität Rostock
Friedrich-Alexander Universität Erlangen-Nürnberg
01. Izmir Institute of Technology
Friedrich-Alexander Universität Erlangen-Nürnberg
Keywords: Bone tissue engineering
Composite scaffolds
Decellularized bone extracellular matrix
Three-dimensional printing
Microbial transglutaminase
Issue Date: Jun-2022
Publisher: Elsevier
Abstract: Three-dimensional (3D) printing technology enables the design of personalized scaffolds with tunable pore size and composition. Combining decellularization and 3D printing techniques provides the opportunity to fabricate scaffolds with high potential to mimic native tissue. The aim of this study is to produce novel decellularized bone extracellular matrix (dbECM)-reinforced composite-scaffold that can be used as a biomaterial for bone tissue engineering. Decellularized bone particles (dbPTs, ∼100 ​μm diameter) were obtained from rabbit femur and used as a reinforcement agent by mixing with gelatin (GEL) in different concentrations. 3D scaffolds were fabricated by using an extrusion-based bioprinter and crosslinking with microbial transglutaminase (mTG) enzyme, followed by freeze-drying to obtain porous structures. Fabricated 3D scaffolds were characterized morphologically, mechanically, and chemically. Furthermore, MC3T3-E1 mouse pre-osteoblast cells were seeded on the dbPTs reinforced GEL scaffolds (GEL/dbPTs) and cultured for 21 days to assess cytocompatibility and cell attachment. We demonstrate the 3D-printability of dbPTs-reinforced GEL hydrogels and the achievement of homogenous distribution of the dbPTs in the whole scaffold structure, as well as bioactivity and cytocompatibility of GEL/dbPTs scaffolds. It was shown that Young's modulus and degradation rate of scaffolds were enhanced with increasing dbPTs content. Multiphoton microscopy imaging displayed the interaction of cells with dbPTs, indicating attachment and proliferation of cells around the particles as well as into the GEL-particle hydrogels. Our results demonstrate that GEL/dbPTs hydrogel formulations have potential for bone tissue engineering.
URI: https://doi.org/10.1016/j.mtbio.2022.100309
https://hdl.handle.net/11147/12390
Appears in Collections:Bioengineering / Biyomühendislik
Chemical Engineering / Kimya Mühendisliği
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

Files in This Item:
File Description SizeFormat 
1-s2.0-S2590006422001077-main.pdfArticle (Makale)6.06 MBAdobe PDFView/Open
Show full item record

CORE Recommender

SCOPUSTM   
Citations

1
checked on Oct 1, 2022

WEB OF SCIENCETM
Citations

1
checked on Oct 1, 2022

Page view(s)

10,256
checked on Sep 26, 2022

Download(s)

2,598
checked on Sep 26, 2022

Google ScholarTM

Check

Altmetric


Items in GCRIS Repository are protected by copyright, with all rights reserved, unless otherwise indicated.