Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/10216
Title: Bi̇yomalzemelerden i̇zole edi̇len Staphylococcus epidermidis suşlarinin yüzey özelli̇kleri̇ni̇n beli̇rlenmesi̇
Other Titles: Investigation of the surface properties of Staphylococcus epidermidis strains isolated from biomaterials
Authors: Sudağıdan, Mert
Erdem, İlker
Çavuşoğlu, Cengiz
Çiftçioğlu, Muhsin
Sudağıdan, Mert
Erdem, İlker
Çiftçioğlu, Muhsin
Izmir Institute of Technology. Biotechnology and Bioengineering Research and Application Center (IZTECH BIYOMER)
Izmir Institute of Technology. Chemical Engineering
Keywords: Atomic force microscopy
Biofilm
Hydrophobicity
Scanning electron microscopy
Staphylococcus epidermidis
Zeta potential
Issue Date: 2010
Publisher: Ankara Mikrobiyoloji Derneği
Abstract: The surface properties of bacteria play an important role on adhesion to the biomaterial surface. In this study, the surface properties of Staphylococcus epidermidis strains isolated from clinically used polymeric biomaterial surfaces were investigated on the basis of zeta potential, hydrophobicity and surface topography. A total of 10 S.epidermidis strains isolated from intravenous catheters (n= 5), endotracheal tubes (n= 3) and central venous catheters (n= 2) which were used in the patients of pulmonary Intensive Care Unit, Ege University Medical Faculty Hospital, were included to the study. Seven of those isolates were biofilm producers, inhabiting biofilm genes, 2 were non-biofilm producers, however, inhabiting biofilm genes, and 1 was non-biofilm producer, inhabiting no biofilm genes. Zeta potential analysis have been performed in 3 different buffers (phosphate-buffered saline, 1 mM potassium chloride and 1 mM potassium phosphate buffer) and at different pH values (pH 4.1-8.2), in order to simulate in vivo environment of the biomaterials. Hydrophobicities of the strains were examined by bacterial adhesion to hydrocarbon (BATH) test and the surface topography of biofilms and slime layers were visualized by atomic force microscopy (AFM) and scanning electron microscopy (SEM) methods. It was found that all strains have negative zeta potential values (surface charge) in all buffers and pH values. In hydrophobicity analysis, the highest value (86%) was determined for non-biofilm forming S.epidermidis strain YT-169b (endotracheal tube isolate) and the lowest hydrophobicity (2.5%) was determined for biofilm forming S.epidermidis strain YT-212 (central venous catheter isolate). Biofilm and slime layers of the strains were imaginated by AFM and SEM analysis in ?m scale. SEM analysis showed that bacteria highly adhered to rough surfaces on biomaterial surfaces and the produced slime layers covered the surface of bacteria. In conclusion, elucidating the surface properties of opportunistic pathogens in different physiologic buffers will give important clues for the production of non-adhesive materials and antibacterial surfaces for those bacteria. It was also estimated that designing the surface of the biomaterial to have negative surface charge in the body and to be as smooth as possible will hamper biofilm formation.
Description: PubMed: 20455404
URI: https://hdl.handle.net/11147/10216
ISSN: 0374-9096
Appears in Collections:Chemical Engineering / Kimya Mühendisliği
IZTECH Research Centers Collection / İYTE Araştırma Merkezleri Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
TR Dizin İndeksli Yayınlar / TR Dizin Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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