Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/10216
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dc.contributor.authorSudağıdan, Mert-
dc.contributor.authorErdem, İlker-
dc.contributor.authorÇavuşoğlu, Cengiz-
dc.contributor.authorÇiftçioğlu, Muhsin-
dc.date.accessioned2021-01-24T18:33:02Z-
dc.date.available2021-01-24T18:33:02Z-
dc.date.issued2010-
dc.identifier.issn0374-9096-
dc.identifier.urihttps://hdl.handle.net/11147/10216-
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/101849-
dc.descriptionPubMed: 20455404en_US
dc.description.abstractThe surface properties of bacteria play an important role on adhesion to the biomaterial surface. In this study, the surface properties of Staphylococcus epidermidis strains isolated from clinically used polymeric biomaterial surfaces were investigated on the basis of zeta potential, hydrophobicity and surface topography. A total of 10 S.epidermidis strains isolated from intravenous catheters (n= 5), endotracheal tubes (n= 3) and central venous catheters (n= 2) which were used in the patients of pulmonary Intensive Care Unit, Ege University Medical Faculty Hospital, were included to the study. Seven of those isolates were biofilm producers, inhabiting biofilm genes, 2 were non-biofilm producers, however, inhabiting biofilm genes, and 1 was non-biofilm producer, inhabiting no biofilm genes. Zeta potential analysis have been performed in 3 different buffers (phosphate-buffered saline, 1 mM potassium chloride and 1 mM potassium phosphate buffer) and at different pH values (pH 4.1-8.2), in order to simulate in vivo environment of the biomaterials. Hydrophobicities of the strains were examined by bacterial adhesion to hydrocarbon (BATH) test and the surface topography of biofilms and slime layers were visualized by atomic force microscopy (AFM) and scanning electron microscopy (SEM) methods. It was found that all strains have negative zeta potential values (surface charge) in all buffers and pH values. In hydrophobicity analysis, the highest value (86%) was determined for non-biofilm forming S.epidermidis strain YT-169b (endotracheal tube isolate) and the lowest hydrophobicity (2.5%) was determined for biofilm forming S.epidermidis strain YT-212 (central venous catheter isolate). Biofilm and slime layers of the strains were imaginated by AFM and SEM analysis in ?m scale. SEM analysis showed that bacteria highly adhered to rough surfaces on biomaterial surfaces and the produced slime layers covered the surface of bacteria. In conclusion, elucidating the surface properties of opportunistic pathogens in different physiologic buffers will give important clues for the production of non-adhesive materials and antibacterial surfaces for those bacteria. It was also estimated that designing the surface of the biomaterial to have negative surface charge in the body and to be as smooth as possible will hamper biofilm formation.en_US
dc.language.isotren_US
dc.publisherAnkara Mikrobiyoloji Derneğien_US
dc.relation.ispartofMikrobiyoloji Bultenien_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAtomic force microscopyen_US
dc.subjectBiofilmen_US
dc.subjectHydrophobicityen_US
dc.subjectScanning electron microscopyen_US
dc.subjectStaphylococcus epidermidisen_US
dc.subjectZeta potentialen_US
dc.titleBi̇yomalzemelerden i̇zole edi̇len Staphylococcus epidermidis suşlarinin yüzey özelli̇kleri̇ni̇n beli̇rlenmesi̇en_US
dc.title.alternativeInvestigation of the surface properties of Staphylococcus epidermidis strains isolated from biomaterials-
dc.typeArticleen_US
dc.institutionauthorSudağıdan, Mert-
dc.institutionauthorErdem, İlker-
dc.institutionauthorÇiftçioğlu, Muhsin-
dc.departmentİzmir Institute of Technology. Biotechnology and Bioengineering Research and Application Center (IZTECH BIYOMER)en_US
dc.departmentİzmir Institute of Technology. Chemical Engineering-
dc.identifier.volume44en_US
dc.identifier.issue1en_US
dc.identifier.startpage93en_US
dc.identifier.endpage103en_US
dc.identifier.wosWOS:000275480700012en_US
dc.identifier.scopus2-s2.0-77749341545en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.pmid20455404en_US
dc.identifier.trdizinid101849en_US
dc.identifier.wosqualityQ4-
dc.identifier.scopusqualityN/A-
item.openairetypeArticle-
item.fulltextWith Fulltext-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextopen-
item.cerifentitytypePublications-
item.languageiso639-1tr-
crisitem.author.dept03.02. Department of Chemical Engineering-
Appears in Collections:Chemical Engineering / Kimya Mühendisliği
IZTECH Research Centers Collection / İYTE Araştırma Merkezleri Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
TR Dizin İndeksli Yayınlar / TR Dizin Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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