Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/9582
Title: Characterization of long living yeast deletion mutants that lack mitochondrial metabolism genes DSS1, PPA2 and AFG3
Authors: Muid, Khandaker Ashfaqul
Kimyon, Önder
Reza, Shahadat Hasan
Karakaya, Hüseyin Çağlar
Koç, Ahmet
Keywords: Longevity
Aging
Mitochondria
ROS
Respiration
Retrograde signaling
PPA2
DSS1
AFG3
Issue Date: 2019
Publisher: Elsevier Ltd.
Abstract: Molecular mechanisms of aging and longevity are still mostly unknown. Mitochondria play central roles in cellular metabolism and aging. In this study, we identified three deletion mutants of mitochondrial metabolism genes (ppa2 Delta, dss1 Delta, and afg3 Delta) that live longer than wild-type cells. These long-lived cells harbored significantly decreased amount of mitochondria] DNA (mtDNA) and reactive oxygen species (ROS). Compared to the serpentine nature of wild-type mitochondria, a different dynamics and distribution pattern of mitochondria were observed in the mutants. Both young and old long-lived cells produced relatively low but adequate levels of ATP for cellular activities. The status of the retrograde signaling was checked by expression of CIT2 gene and found activated in long-lived mutants. The mutant cells were also profiled for their gene expression patterns, and genes that were differentially regulated were determined. All long-lived cells comprised similar pleiotropic phenotype regarding mitochondrial dynamics and functions. Thus, this study suggests that DSS1, PPA2, and AFG3 genes modulate the lifespan by altering the mitochondrial morphology and functions.
Description: WOS: 000472241500022
PubMed: 31082499
URI: https://doi.org/10.1016/j.gene.2019.05.001
https://hdl.handle.net/11147/9582
ISSN: 0378-1119
1879-0038
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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