Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/9556
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dc.contributor.authorAmmanath, Gopal-
dc.contributor.authorYeasmi, Sanjida-
dc.contributor.authorSrinivasulu, Yuvasri-
dc.contributor.authorVats, Mukti-
dc.contributor.authorCheema, Jamal Ahmed-
dc.contributor.authorNabilah, Fairuz-
dc.contributor.authorLiedberg, Bo-
dc.contributor.authorYıldız, Ümit Hakan-
dc.date.accessioned2020-07-25T22:16:55Z-
dc.date.available2020-07-25T22:16:55Z-
dc.date.issued2019-
dc.identifier.issn0003-2670-
dc.identifier.issn1873-4324-
dc.identifier.urihttps://doi.org/10.1016/j.aca.2019.03.036-
dc.identifier.urihttps://hdl.handle.net/11147/9556-
dc.descriptionWOS: 000465212100011en_US
dc.descriptionPubMed: 31027524en_US
dc.description.abstractA flow-through colorimetric assay for detection of nucleic acids in plasma is reported. The proposed assay features an array of four polyvinylidene fluoride (PVDF) membranes impregnated with cationic poly (3-alkoxy-4-methylthiophene) (PT) as an optical reporter. The sensing strategy is based on monitoring the changes in optical properties of PT, upon complexation with target nucleic acids in the presence and in the absence of their corresponding complementary peptide nucleic acids (PNAs). As a proof of concept, the proposed methodology is validated using two biomarkers; lung cancer associated microRNA (mir21) and hepatitis B virus DNA (HBV-DNA). The flow-through colorimetric assay enabled detection of mir21 and HBV-DNA in plasma without requiring tedious sample pre-treatment and clean up protocols. Colorimetric responses for mir21 and HBV-DNA were obtained at nanomolar concentrations over five orders of magnitudes (from 1 nM to 10 mu M), with a limit of detection of -0.6 nM and -2 nM in DI water and plasma, respectively. A logic gate system was developed to utilize the colorimetric assay responses as inputs for discrimination of mir21 and HBV-DNA and subsequently to obtain a profile of nucleic acids in samples that exceed respective clinical threshold limits, thereby enabling rapid and point of care (POC) disease diagnosis. Furthermore, the proposed methodology can be utilized for detection of a large number of nucleic acids in plasma by extending the array of PT impregnated membranes incorporated with their corresponding complementary PNAs. (C) 2019 Elsevier B.V. All rights reserved.en_US
dc.language.isoenen_US
dc.publisherElsevier Ltd.en_US
dc.relation.ispartofAnalytica Chimica Actaen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectColorimetric arrayen_US
dc.subjectPolythiopheneen_US
dc.subjectPlasmaen_US
dc.subjectNucleic acidsen_US
dc.subjectmir21en_US
dc.subjectHBV-DNAen_US
dc.titleFlow-through colorimetric assay for detection of nucleic acids in plasmaen_US
dc.typeArticleen_US
dc.departmentIzmir Institute of Technology. Chemistryen_US
dc.identifier.volume1066en_US
dc.identifier.startpage102en_US
dc.identifier.endpage111en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.cont.department-temp[Ammanath, Gopal; Yeasmi, Sanjida; Srinivasulu, Yuvasri; Cheema, Jamal Ahmed; Nabilah, Fairuz; Alagappan, Palaniappan; Liedberg, Bo] Nanyang Technol Univ, Ctr Biomimet Sensor Sci, Singapore 637553, Singapore; [Ammanath, Gopal] Nanyang Technol Univ, Interdisciplinary Grad Sch, NTU Inst Hlth Technol, Singapore 637553, Singapore; [Ammanath, Gopal; Yeasmi, Sanjida; Srinivasulu, Yuvasri; Cheema, Jamal Ahmed; Nabilah, Fairuz; Alagappan, Palaniappan; Liedberg, Bo] Nanyang Technol Univ, Sch Mat Sci & Engn, Singapore 639798, Singapore; [Vats, Mukti; Srivastava, Rohit] Indian Inst Technol, Dept Biosci & Bioengn, Mumbai 400076, Maharashtra, India; [Yildiz, Umit Hakan] Izmir Inst Technol, Dept Chem, TR-35430 Izmir, Turkeyen_US
dc.identifier.doi10.1016/j.aca.2019.03.036-
dc.relation.doi10.1016/j.aca.2019.03.036en_US
dc.coverage.doi10.1016/j.aca.2019.03.036en_US
item.fulltextNo Fulltext-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeArticle-
item.languageiso639-1en-
item.cerifentitytypePublications-
item.grantfulltextnone-
crisitem.author.deptDepartment of Chemistry-
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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