Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/9304
Title: In vitro evaluation of Spirulina platensis extract incorporated skin cream with its wound healing and antioxidant activities
Authors: Güneş, Seda
Tamburacı, Sedef
Conk Dalay, Meltem
Deliloğlu Gürhan, İsmet
Keywords: Cyanobacteria
Crude extract
Phycocyanin
Cytotoxicity
Genotoxicity
Wound scratch assay
Publisher: Taylor & Francis
Abstract: Context: Algae have gained importance in cosmeceutical product development due to their beneficial effects on skin health and therapeutical value with bioactive compounds. Spirulina platensis Parachas (Phormidiaceae) is renowned as a potential source of high-value chemicals and recently used in skincare products. Objective: This study develops and evaluates skin creams incorporated with bioactive S. platensis extract. Materials and methods:Spirulina platensis was cultivated, the aqueous crude extract was prepared and in vitro cytotoxicity of S. platensis extract in the range of 0.001-1% concentrations for 1, 3 and 7d on HS2 keratinocyte cells was determined. Crude extracts were incorporated in skin cream formulation at 0.01% (w/w) concentration and in vitro wound healing and genotoxicity studies were performed. Immunohistochemical staining was performed to determine the collagen activity. Results: 0.1% S. platensis extract exhibited higher proliferation activity compared with the control group with 198% of cell viability after 3 d. Skin cream including 1.125% S. platensis crude extract showed enhanced wound healing effect on HS2 keratinocyte cell line and the highest HS2 cell viability % was obtained with this concentration. The micronucleus (MN) assay results indicated that S. platensis extract incorporated creams had no genotoxic effect on human peripheral blood cells. Immunohistochemical analysis showed that collagen 1 immunoreactivity was improved by increased extract concentration and it was strongly positive in cells treated with 1.125% extract incorporated skin cream. Conclusions: The cell viability, wound healing activity and genotoxicity results showed that S. platensis incorporated skin cream could be of potential value in cosmeceutical and biomedical applications.
URI: https://doi.org/10.1080/13880209.2017.1331249
https://hdl.handle.net/11147/9304
ISSN: 1388-0209
1744-5116
Appears in Collections:Bioengineering / Biyomühendislik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

Files in This Item:
File SizeFormat 
In vitro evaluation.pdf1.65 MBAdobe PDFView/Open
Show full item record



CORE Recommender

SCOPUSTM   
Citations

67
checked on Jul 12, 2024

WEB OF SCIENCETM
Citations

54
checked on Jul 13, 2024

Page view(s)

446
checked on Jul 15, 2024

Download(s)

118
checked on Jul 15, 2024

Google ScholarTM

Check




Altmetric


Items in GCRIS Repository are protected by copyright, with all rights reserved, unless otherwise indicated.