Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/9275
Title: Bacterial detection using bacteriophages and gold nanorods by following time-dependent changes in Raman spectral signals
Authors: Moghtader, Farzaneh
Tomak, Aysel
Zareie, Hadi M.
Pişkin, Erhan
Keywords: Bacterial detection
Escherichia coli
Gold nanorods
T4-bacteriophages
Raman spectral analysis
Time dependent
Issue Date: 2018
Publisher: Informa Healthcare
Abstract: This study attemps to develop bacterial detection strategies using bacteriophages and gold nanorods (GNRs) by Raman spectral analysis. Escherichia coli was selected as the target and its specific phage was used as the bioprobe. Target bacteria and phages were propagated/purified by traditional techniques. GNRs were synthesized by using hexadecyltrimethyl ammonium bromide (CTAB) as stabilizer. A two-step detection strategy was applied: Firstly, the target bacteria were interacted with GNRs in suspensions, and then they were dropped onto silica substrates for detection. It was possible to obtain clear surface-enchanced Raman spectroscopy (SERS) peaks of the target bacteria, even without using phages. In the second step, the phage nanoemulsions were droped onto the bacterial-GNRs complexes on those surfaces and time-dependent changes in the Raman spectra were monitored at different time intervals upto 40 min. These results demonstrated that how one can apply phages with plasmonic nanoparticles for detection of pathogenic bacteria very effectively in a quite simple test.
URI: https://doi.org/10.1080/21691401.2018.1452251
https://hdl.handle.net/11147/9275
ISSN: 2169-1401
2169-141X
Appears in Collections:Materials Science and Engineering / Malzeme Bilimi ve Mühendisliği
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

Files in This Item:
File SizeFormat 
Bacterial detection.pdf2.34 MBAdobe PDFView/Open
Show full item record

CORE Recommender

SCOPUSTM   
Citations

2
checked on Oct 1, 2022

WEB OF SCIENCETM
Citations

5
checked on Oct 1, 2022

Page view(s)

76
checked on Oct 3, 2022

Download(s)

12
checked on Oct 3, 2022

Google ScholarTM

Check

Altmetric


Items in GCRIS Repository are protected by copyright, with all rights reserved, unless otherwise indicated.