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Title: Bacterial detection using bacteriophages and gold nanorods by following time-dependent changes in Raman spectral signals
Authors: Moghtader, Farzaneh
Tomak, Aysel
Zareie, Hadi M.
Piskin, Erhan
Tomak, Aysel
Izmir Institute of Technology. Materials Science and Engineering
Keywords: Bacterial detection
Escherichia coli
gold nanorods
Raman spectral analysis
time dependent
Issue Date: 2018
Publisher: Informa Healthcare
Abstract: This study attemps to develop bacterial detection strategies using bacteriophages and gold nanorods (GNRs) by Raman spectral analysis. Escherichia coli was selected as the target and its specific phage was used as the bioprobe. Target bacteria and phages were propagated/purified by traditional techniques. GNRs were synthesized by using hexadecyltrimethyl ammonium bromide (CTAB) as stabilizer. A two-step detection strategy was applied: Firstly, the target bacteria were interacted with GNRs in suspensions, and then they were dropped onto silica substrates for detection. It was possible to obtain clear surface-enchanced Raman spectroscopy (SERS) peaks of the target bacteria, even without using phages. In the second step, the phage nanoemulsions were droped onto the bacterial-GNRs complexes on those surfaces and time-dependent changes in the Raman spectra were monitored at different time intervals upto 40 min. These results demonstrated that how one can apply phages with plasmonic nanoparticles for detection of pathogenic bacteria very effectively in a quite simple test.
Description: WOS: 000459181400012
PubMed: 29583029
ISSN: 2169-1401
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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