Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/9133
Full metadata record
DC FieldValueLanguage
dc.contributor.authorKüçükköse, Cansu-
dc.contributor.authorYalçın Özuysal, Özden-
dc.date.accessioned2020-07-25T22:07:26Z-
dc.date.available2020-07-25T22:07:26Z-
dc.date.issued2019-
dc.identifier.issn1300-0152-
dc.identifier.issn1303-6092-
dc.identifier.urihttps://doi.org/10.3906/biy-1808-58-
dc.identifier.urihttps://hdl.handle.net/11147/9133-
dc.descriptionPubMed: 30930637en_US
dc.description.abstractMetastasis is the main reason for death in breast cancer. Understanding the molecular players in metastasis is crucial for diagnostic and therapeutic purposes. Notch signalling plays an oncogenic role in breast tumorigenesis and is involved in metastasis. Downstream mediators of Notch signalling in prometastatic processes are not yet fully discovered. Here we aimed to investigate whether Notch signalling regulates the expression of SEMA3C, HMGA2, CXCL14, CXCR7, and CCL20, which are involved in prometastatic processes, in breast cell lines. To this end, expression of the selected genes was analysed following Notch activation by overexpression of the Notch1 intracellular domain in the normal breast epithelial cell line MCF10A, and inhibition by silencing of the Notch transcriptional mediator RBPj kappa in the breast cancer cell line MDA MB 231. SEMA3C and HMGA2 mRNA were decreased, while CXCL14 and CXCR7 mRNA were increased significantly in response to Notch activation in MCF10A cells. Notch inhibition in MDA MB 231 cells significantly decreased HMGA2 and CCL20 mRNA. Protein levels were not significantly altered by Notch modulation. In conclusion, we showed that Notch signalling regulates expression of SEMA3C, CXCL14, CCL20, CXCR7, and HMGA2, which are prominent candidate genes that might function downstream of Notch to induce prometastatic processes.en_US
dc.language.isoenen_US
dc.publisherTÜBİTAKen_US
dc.relation.ispartofTurkish Journal of Biologyen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBreast canceren_US
dc.subjectMetastasisen_US
dc.subjectNotch signallingen_US
dc.subjectSEMA3Cen_US
dc.subjectHMGA2en_US
dc.subjectCXCL14en_US
dc.subjectCXCR7en_US
dc.subjectCCL20en_US
dc.titleEffects of Notch signalling on the expression of SEMA3C, HMGA2, CXCL14, CXCR7, and CCL20 in breast canceren_US
dc.typeArticleen_US
dc.authorid0000-0003-0552-368X-
dc.institutionauthorKüçükköse, Cansu-
dc.institutionauthorYalçın Özuysal, Özden-
dc.departmentIzmir Institute of Technology. Molecular Biology and Geneticsen_US
dc.identifier.volume43en_US
dc.identifier.issue1en_US
dc.identifier.startpage70en_US
dc.identifier.endpage76en_US
dc.identifier.wosWOS: 000458059900008-
dc.identifier.scopusSCOPUS:2-s2.0-85062663993-
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.3906/biy-1808-58-
dc.relation.doi10.3906/biy-1808-58en_US
dc.coverage.doi10.3906/biy-1808-58en_US
item.cerifentitytypePublications-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeArticle-
item.grantfulltextopen-
item.languageiso639-1en-
item.fulltextWith Fulltext-
crisitem.author.deptDepartment of Molecular Biology and Genetics-
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
Files in This Item:
File SizeFormat 
biy-43-1-8-1808-58.pdf3.49 MBAdobe PDFView/Open
Show simple item record

CORE Recommender

SCOPUSTM   
Citations

4
checked on Dec 4, 2021

WEB OF SCIENCETM
Citations

6
checked on Dec 4, 2021

Page view(s)

28
checked on Dec 8, 2021

Download(s)

2
checked on Dec 8, 2021

Google ScholarTM

Check

Altmetric


Items in GCRIS Repository are protected by copyright, with all rights reserved, unless otherwise indicated.