Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/9079
Title: Self-assembly behavior of the keratose proteins extracted from oxidized Ovis aries wool fibers
Authors: Pakkaner, Efecan
Yalçın, Damla
Uysal, Berk
Top, Ayben
Keywords: Wool keratose
Self-assembly
Hydrogel
Issue Date: 2019
Publisher: Elsevier Ltd.
Abstract: Water soluble keratose proteins were obtained from an Ovis Aries wool using peracetic acid oxidation. The wool samples and the extracted keratose proteins were characterized by using FTIR, XRD, SEM and TGA techniques. Fractions of alpha-keratose (MW = 43-53 kDa) along with protein species with molecular weights between 23 kDa and 33 kDa were identified in the SDS-PAGE analysis result of the extracted protein mixture. DLS and AFM experiments indicated that self-assembled globular nanoparticles with diameters between 15 nm and 100 nm formed at 5 mg/ml keratose concentration. On the other hand, upon incubation of 10 w % keratose solutions at 37 degrees C and 50 degrees C, interconnected keratose hydrogels with respective storage modulus (G') values of 0.17 +/- 0.03 kPa and 3.7 +/- 0.5 kPa were obtained. It was shown that the keratose hydrogel prepared at 37 degrees C supported L929 mouse fibroblast cell proliferation which suggested that these keratose hydrogels could be promising candidates in soft tissue engineering applications. (C) 2018 Elsevier B.V. All rights reserved.
Description: PubMed: 30572050
URI: https://doi.org/10.1016/j.ijbiomac.2018.12.129
https://hdl.handle.net/11147/9079
ISSN: 0141-8130
1879-0003
Appears in Collections:Chemical Engineering / Kimya Mühendisliği
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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