Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/8911
Title: Microbial transformation of cycloastragenol and astragenol by endophytic fungi isolated from astragalus species
Authors: Ekiz, Güner
Yılmaz, Sinem
Yusufoğlu, Hasan
Ballar Kırmızıbayrak, Petek
Bedir, Erdal
Issue Date: 2019
Publisher: American Chemical Society
Abstract: Biotransformation of Astragalus sapogenins (cycloastragenol (1) and astragenol (2)) by Astragalus species originated endophytic fungi resulted in the production of five new metabolites (3, 7, 10, 12, 14) together with 10 known compounds. The structures of the new compounds were established by NMR spectroscopic and HRMS analysis. Oxygenation, oxidation, epoxidation, dehydrogenation, and ring cleavage reactions were observed on the cycloartane (9,19-cyclolanostane) nucleus. The ability of the compounds to increase telomerase activity in neonatal cells was also evaluated. After prescreening studies to define potent telomerase activators, four compounds were selected for subsequent bioassays. These were performed using very low doses ranging from 0.1 to 30 nM compared to the control cells treated with DMSO. The positive control cycloastragenol and 8 were found to be the most active compounds, with 5.2- (2 nM) and 5.1- (0.5 nM) fold activations versus DMSO, respectively. At the lowest dose of 0.1 nM, compounds 4 and 13 provided 3.5- and 3.8-fold activations, respectively, while cycloastragenol showed a limited activation (1.5-fold).
Description: PubMed: 31713424
URI: https://doi.org/10.1021/acs.jnatprod.9b00336
https://hdl.handle.net/11147/8911
ISSN: 0163-3864
1520-6025
Appears in Collections:Bioengineering / Biyomühendislik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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