Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/7606
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dc.contributor.authorTürker, Esra-
dc.contributor.authorDemirçak, Nida-
dc.contributor.authorArslan Yıldız, Ahu-
dc.date.accessioned2020-01-21T08:11:24Z-
dc.date.available2020-01-21T08:11:24Z-
dc.date.issued2018-07en_US
dc.identifier.citationTürker, E., Demirçak, N., and Arslan Yıldız, A. (2018). Scaffold-free three-dimensional cell culturing using magnetic levitation. Biomaterials Science, 6(7), 1745-1753. doi:10.1039/c8bm00122gen_US
dc.identifier.issn2047-4830-
dc.identifier.issn2047-4830-
dc.identifier.issn2047-4849-
dc.identifier.urihttps://doi.org/10.1039/C8BM00122G-
dc.identifier.urihttps://hdl.handle.net/11147/7606-
dc.description.abstractThree-dimensional (3D) cell culture has emerged as a pioneering methodology and is increasingly utilized for tissue engineering, 3D bioprinting, cancer model studies and drug development studies. The 3D cell culture methodology provides artificial and functional cellular constructs serving as a modular playground prior to animal model studies, which saves substantial efforts, time and experimental costs. The major drawback of current 3D cell culture methods is their dependency on biocompatible scaffolds, which often require tedious syntheses and fabrication steps. Herein, we report an easy-to-use methodology for the formation of scaffold-free 3D cell culture and cellular assembly via magnetic levitation in the presence of paramagnetic agents. To paramagnetize the cell culture environment, three different Gadolinium(iii) chelates were utilized, which led to levitation and assembly of cells at a certain levitation height. The assembly and close interaction of cells at the levitation height where the magnetic force was equilibrated with gravitational force triggered the formation of complex 3D cellular structures. It was shown that Gd(iii) chelates provided an optimal levitation that induced intercellular interactions in scaffold-free format without compromising cell viability. NIH 3T3 mouse fibroblasts and HCC827 non-small-cell lung cancer cells were evaluated via the magnetic levitation system, and the formation of 3D cell culture models was validated for both cell lines. Hereby, the developed magnetic levitation system holds promises for complex cellular assemblies and 3D cell culture studies.en_US
dc.description.sponsorshipIYTE BAP; 2016IYTE70en_US
dc.language.isoenen_US
dc.publisherRoyal Society of Chemistryen_US
dc.relation.ispartofBiomaterials Scienceen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCell cultureen_US
dc.subjectMagnetic levitationen_US
dc.subject3D cellular structureen_US
dc.subjectBiocompatible scaffoldsen_US
dc.subjectGadolinium compoundsen_US
dc.titleScaffold-free three-dimensional cell culturing using magnetic levitationen_US
dc.typeArticleen_US
dc.authorid0000-0003-0348-0575en_US
dc.institutionauthorTürker, Esra-
dc.institutionauthorDemirçak, Nida-
dc.institutionauthorArslan Yıldız, Ahu-
dc.departmentİzmir Institute of Technology. Bioengineeringen_US
dc.identifier.volume6en_US
dc.identifier.issue7en_US
dc.identifier.startpage1745en_US
dc.identifier.endpage1753en_US
dc.identifier.wosWOS:000447710700007en_US
dc.identifier.scopus2-s2.0-85049070854en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1039/c8bm00122g-
dc.identifier.pmid29700506en_US
dc.relation.doi10.1039/c8bm00122gen_US
dc.coverage.doi10.1039/c8bm00122gen_US
dc.identifier.wosqualityQ1-
dc.identifier.scopusqualityQ1-
item.grantfulltextopen-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.openairetypeArticle-
item.languageiso639-1en-
item.fulltextWith Fulltext-
crisitem.author.dept01.01. Units Affiliated to the Rectorate-
crisitem.author.dept03.01. Department of Bioengineering-
Appears in Collections:Bioengineering / Biyomühendislik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Sürdürülebilir Yeşil Kampüs Koleksiyonu / Sustainable Green Campus Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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