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Title: An answer to colon cancer treatment by mesenchymal stem cell originated from adipose tissue
Authors: İplik, Elif Sinem
ERtuğrul, Barış
Kozanoğlu, İlknur
Baran, Yusuf
Keywords: Apoptosis
Cell death
Colon cancer
Stem cells
Issue Date: May-2018
Publisher: Mashhad University of Medical Sciences
Source: İplik, E. S., Ertuğrul, B., Kozanoğlu, İ., and Baran, Y. (2018). An answer to colon cancer treatment by mesenchymal stem cell originated from adipose tissue. Iranian Journal of Basic Medical Sciences, 21(5), 465-468. doi:10.22038/IJBMS.2018.26152.6420
Abstract: Objective(s): Colon cancer is risen up with its complex mechanism that directly impacts on its treatment as well as its common prevalence. Mesenchymal stem cells (MSCs) have been considered as a therapeutic candidate for conventional disease including cancer. In this research, we have focused on apoptotic effects of adipose tissue-derived MSCs in colon cancer. Materials and Methods: MSCs were obtained from adipose tissue and characterized by Flowcytometer using suitable antibodies. MSCs, HT-29, HCT-116, RKO and healthy cell line MRC5 were cultured by different seeding procedure. After cell viability assay, changes in caspase 3 enzyme activity and the level of phosphatidylserine were measured. Results: For cell viability assay, a 48 hr incubation period was chosen to seed all cells together. There was a 1.36-fold decrease in caspase 3 enzyme activity by co-treatment of RKO and MSCs in addition to 2.02-fold decrease in HT-29 and MSCs co-treatment, and 1.103-fold increase in HCT-116 and MSCs. The results demonstrated that HCT-116 led to the highest rate of apoptotic cell death (7.5%) compared with other cells. Conclusion: We suggest that MSCs might remain a new treatment option for cancer by its differentiation and repair capacity.
ISSN: 2008-3866
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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