Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/7550
Title: Luminescent device for the detection of oxidative stress biomarkers in artificial urine
Authors: Ammanath, Gopal
Yıldız, Ümit Hakan
Palaniappan, Alagappan
Liedberg, Bo
Keywords: Naked eye detection
Biomarkers
Polythiophene
Artificial urine
Oxidative stress
Deoxyguanosine
Issue Date: Mar-2018
Publisher: American Chemical Society
Source: Ammanath, G., Yıldız, Ü. H., Palaniappan, A., and Liedberg, B. (2018). Luminescent device for the detection of oxidative stress biomarkers in artificial urine. ACS Applied Materials and Interfaces, 10(9), 7730-7736. doi:10.1021/acsami.7b17252
Abstract: A luminescent paper-based device for the visual detection of oxidative stress biomarkers is reported. The device consists of a polyvinylidene fluoride membrane impregnated with poly(3-alkoxy-4-methylthiophene) (PT) for colorimetric detection. 8-hydroxy-2′-deoxyguanosine (8-OHdG), a biomarker associated with oxidative stress, is used as a model system for validating the proposed methodology. The detection strategy is based on monitoring the changes in optical properties of PT associated with its conformational changes upon interaction with an aptamer in the presence and in the absence of 8-OHdG. Fluorometric and colorimetric monitoring revealed linear responses for 8-OHdG concentrations between 50 pM and 500 nM (∼14 pg/mL to 140 ng/mL), with limits of detection of ∼300 pM and ∼350 pM, respectively for (n = 3). Colorimetric responses in artificial urine ascertained rapid, sensitive, and selective detection of 8-OHdG at clinically relevant (pM to nM) concentration levels. Furthermore, the proposed methodology enables point-of-care diagnostics for oxidative stress without requiring sophisticated instrumentation.
URI: https://doi.org/10.1021/acsami.7b17252
https://hdl.handle.net/11147/7550
ISSN: 1944-8244
1944-8244
1944-8252
Appears in Collections:Chemistry / Kimya
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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