Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/7072
Title: Deep sequencing reveals two Jurkat subpopulations with distinct miRNA profiles during camptothecin-induced apoptosis
Authors: Erdoğan, İpek
Coşacak, Mehmet İlyas
Nalbant, Ayten
Akgül, Bünyamin
Erdoğan, İpek
Coşacak, Mehmet İlyas
Nalbant, Ayten
Akgül, Bünyamin
Izmir Institute of Technology. Molecular Biology and Genetics
Keywords: Apoptosis
Deep sequencing
Jurkat
MicroRNAs
Issue Date: 2018
Publisher: TUBITAK
Source: Erdoğan, İ., CoşacaK, M. İ., Nalbant, A., and Akgül, B. (2018). Deep sequencing reveals two Jurkat subpopulations with distinct miRNA profiles during camptothecin-induced apoptosis. Turkish Journal of Biology, 42(2), 113-122. doi:10.3906/biy-1710-62
Abstract: MicroRNAs (miRNAs) are small noncoding RNAs of about 19-25 nt that regulate gene expression posttranscriptionally under various cellular conditions, including apoptosis. The miRNAs involved in modulation of apoptotic events in T cells are partially known. However, heterogeneity associated with cell lines makes it difficult to interpret gene expression signatures, especially in cancer-related cell lines. Treatment of the Jurkat T-cell leukemia cell line with the universal apoptotic drug, camptothecin, resulted in identification of two Jurkat subpopulations: one that is sensitive to camptothecin and another that is rather intrinsically resistant. We sorted apoptotic Jurkat cells from nonapoptotic ones prior to profiling miRNAs through deep sequencing. Our data showed that a total of 184 miRNAs were dysregulated. Interestingly, the apoptotic and nonapoptotic subpopulations exhibited distinct miRNA expression profiles. In particular, 6 miRNAs were inversely expressed in these two subpopulations. The pyrosequencing results were validated by real-time qPCR. Altogether, these results suggest that miRNAs modulate apoptotic events in T cells and that cellular heterogeneity requires careful interpretation of miRNA expression profiles obtained from drug-treated cell lines.
URI: http://doi.org/10.3906/biy-1710-62
http://hdl.handle.net/11147/7072
ISSN: 1300-0152
1300-0152
1303-6092
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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