Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/7069
Title: PEG-peptide conjugate containing cathepsin B degradation unit as a doxorubicin carrier system
Authors: Şentürk, Nesligül
Top, Ayben
Şentürk, Nesligül
Top, Ayben
Izmir Institute of Technology. Chemical Engineering
Keywords: Cathepsin B
Controlled release
Doxorubicin
Polymer conjugates
Issue Date: 2018
Publisher: TUBITAK
Source: Şentürk, N., and Top, A. (2018). PEG-peptide conjugate containing cathepsin B degradation unit as a doxorubicin carrier system. Turkish Journal of Chemistry, 42(2), 385-400. doi:10.3906/kim-1706-65
Abstract: A drug delivery system (DDS) containing a cathepsin B degradable sequence and pH-responsive histidines was prepared by methoxypolyethylene glycol and peptide conjugation. Doxorubicin was attached to the carrier system using amide linkage to give the final form of the DDS, denoted as mPEG-AT3-DOX. mPEG-AT3-DOX exhibited a bimodal size distribution at about 15 and 30 nm independent of pH, whereas the size of the control DDS containing no peptide sequence, mPEG-DOX, was measured as ∼ 15–20 nm. At the end of 72 h, % doxorubicin release from both of the DDSs was observed to be below 8.5 ± 3% in the absence of cathepsin B, and it increased to 17 ± 2% in the presence of cathepsin B for mPEG-AT3-DOX. Complete degradation of AT3 peptide within 3 h upon incubation with cathepsin B suggests that lower than expected doxorubicin release is likely due to the aggregation tendency of mPEG-AT3-DOX. Absolute IC50 values indicated that the cytotoxicity trend of the samples is in the order of free DOX ≥ mPEG-AT3-DOX >mPEG-DOX. Considering these results, PEG-peptide-doxorubicin conjugates can be promising candidates in cancer therapy if they are designed to have more pronounced pH-responsive behavior to increase the drug release rate.
URI: http://doi.org/10.3906/kim-1706-65
http://hdl.handle.net/11147/7069
ISSN: 1300-0527
1303-6130
Appears in Collections:Chemical Engineering / Kimya Mühendisliği
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

Files in This Item:
File Description SizeFormat 
7069.pdfMakale (Article)1.86 MBAdobe PDFThumbnail
View/Open
Show full item record

CORE Recommender

SCOPUSTM   
Citations

2
checked on Jun 12, 2021

WEB OF SCIENCETM
Citations

3
checked on Jun 12, 2021

Google ScholarTM

Check

Altmetric


Items in GCRIS Repository are protected by copyright, with all rights reserved, unless otherwise indicated.