Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/6976
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dc.contributor.authorAdan, Aysun-
dc.contributor.authorKiraz, Yağmur-
dc.contributor.authorBaran, Yusuf-
dc.date.accessioned2018-11-16T06:58:57Z-
dc.date.available2018-11-16T06:58:57Z-
dc.date.issued2016-11-
dc.identifier.citationAdan, A., Kiraz, Y., and Baran, Y. (2016). Cell proliferation and cytotoxicity assays. Current Pharmaceutical Biotechnology, 17(14), 1213-1221. doi: 10.2174/1389201017666160808160513en_US
dc.identifier.issn1389-2010-
dc.identifier.urihttp://doi.org/10.2174/1389201017666160808160513-
dc.identifier.urihttp://hdl.handle.net/11147/6976-
dc.description.abstractCell viability is defined as the number of healthy cells in a sample and proliferation of cells is a vital indicator for understanding the mechanisms in action of certain genes, proteins and pathways involved cell survival or death after exposing to toxic agents. Generally, methods used to determine viability are also common for the detection of cell proliferation. Cell cytotoxicity and proliferation assays are generally used for drug screening to detect whether the test molecules have effects on cell proliferation or display direct cytotoxic effects. Regardless of the type of cell-based assay being used, it is important to know how many viable cells are remaining at the end of the experiment. There are a variety of assay methods based on various cell functions such as enzyme activity, cell membrane permeability, cell adherence, ATP production, co-enzyme production, and nucleotide uptake activity. These methods could be basically classified into different categories: (I) dye exclusion methods such as trypan blue dye exclusion assay, (II) methods based on metabolic activity, (III) ATP assay, (IV) sulforhodamine B assay, (V) protease viability marker assay, (VI) clonogenic cell survival assay, (VII) DNA synthesis cell proliferation assays and (V) raman micro-spectroscopy. In order to choose the optimal viability assay, the cell type, applied culture conditions, and the specific questions being asked should be considered in detail. This particular review aims to provide an overview of common cell proliferation and cytotoxicity assays together with their own advantages and disadvantages, their methodologies, comparisons and intended purposes.en_US
dc.language.isoenen_US
dc.publisherBentham Science Publishers B.V.en_US
dc.relation.ispartofCurrent Pharmaceutical Biotechnologyen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAlamar blueen_US
dc.subjectCytotoxicityen_US
dc.subjectTetrazoliumen_US
dc.subjectViabilityen_US
dc.titleCell proliferation and cytotoxicity assaysen_US
dc.typeBook Reviewen_US
dc.authoridTR119193en_US
dc.institutionauthorKiraz, Yağmur-
dc.institutionauthorBaran, Yusuf-
dc.departmentIzmir Institute of Technology. Molecular Biology and Geneticsen_US
dc.identifier.volume17en_US
dc.identifier.issue14en_US
dc.identifier.startpage1213en_US
dc.identifier.endpage1221en_US
dc.identifier.wosWOS:000388513800003
dc.identifier.scopusSCOPUS:2-s2.0-84995948183
dc.relation.publicationcategoryDiğeren_US
dc.identifier.doi10.2174/1389201017666160808160513-
dc.relation.doi10.2174/1389201017666160808160513en_US
dc.coverage.doi10.2174/1389201017666160808160513en_US
item.cerifentitytypePublications-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeBook Review-
item.grantfulltextopen-
item.languageiso639-1en-
item.fulltextWith Fulltext-
crisitem.author.deptDepartment of Molecular Biology and Genetics-
crisitem.author.deptDepartment of Molecular Biology and Genetics-
crisitem.author.deptDepartment of Molecular Biology and Genetics-
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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