Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/6811
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dc.contributor.authorBali, Elif Burcu-
dc.contributor.authorErgin, Volkan-
dc.contributor.authorRackova, Lucia-
dc.contributor.authorBayraktar, Oğuz-
dc.contributor.authorKüçükboyacı, Nurgün-
dc.contributor.authorKarasu, Çimen-
dc.date.accessioned2018-02-20T11:31:35Z-
dc.date.available2018-02-20T11:31:35Z-
dc.date.issued2014-
dc.identifier.citationBali, E. B., Ergin, V., Rackova, L., Bayraktar, O., Küçükboyacı, N., and Karasu, Ç. (2014). Olive leaf extracts protect cardiomyocytes against 4-hydroxynonenal-induced toxicity in vitro: Comparison with oleuropein, hydroxytyrosol, and quercetin. Planta Medica, 80(12), 984-992. doi:10.1055/s-0034-1382881en_US
dc.identifier.issn0032-0943-
dc.identifier.issn1439-0221-
dc.identifier.urihttp://doi.org/10.1055/s-0034-1382881-
dc.identifier.urihttp://hdl.handle.net/11147/6811-
dc.description.abstractOlive (Olea europaea) leaf, an important traditional herbal medicine, displays cardioprotection that may be related to the cellular redox modulating effects of its polyphenolic constituents. This study was undertaken to investigate the protective effect of the ethanolic and methanolic extracts of olive leaves compared to the effects of oleuropein, hydroxytyrosol, and quercetin as a positive standard in a carbonyl compound (4-hydroxynonenal)- induced model of oxidative damage to rat cardiomyocytes (H9c2). Cell viability was detected by the MTT assay; reactive oxygen species production was assessed by the 2′,7′-dichlorodihydrofluorescein diacetate method, and the mitochondrial membrane potential was determined using a JC-1 dye kit. Phospho-Hsp27 (Ser82), phospho-MAPKAPK-2 (Thr334), phospho-c-Jun (Ser73), cleaved-caspase-3 (cl-CASP3) (Asp175), and phospho-SAPK/JNK (Thr183/Tyr185) were measured by Western blotting. The ethanolic and methanolic extracts of olive leaves inhibited 4-hydroxynonenal-induced apoptosis, characterized by increased reactive oxygen species production, impaired viability (LD50: 25 μM), mitochondrial dysfunction, and activation of pro-apoptotic cl-CASP3. The ethanolic and methanolic extracts of olive leaves also inhibited 4-hydroxynonenal-induced phosphorylation of stress-activated transcription factors, and the effects of extracts on p-SAPK/JNK, p-Hsp27, and p-MAPKAPK-2 were found to be concentration-dependent and comparable with oleuropein, hydroxytyrosol, and quercetin. While the methanolic extract downregulated 4-hydroxynonenal-induced p-MAPKAPK-2 and p-c-Jun more than the ethanolic extract, it exerted a less inhibitory effect than the ethanolic extract on 4-hydroxynonenal-induced p-SAPK/JNK and p-Hsp27. cl-CASP3 and p-Hsp27 were attenuated, especially by quercetin. Experiments showed a predominant reactive oxygen species inhibitory and mitochondrial protecting ability at a concentration of 1-10 μg/mL of each extract, oleuropein, hydroxytyrosol, and quercetin. The ethanolic extract of olive leaves, which contains larger amounts of oleuropein, hydroxytyrosol, verbascoside, luteolin, and quercetin (by HPLC) than the methanolic one, has more protecting ability on cardiomyocyte viability than the methanolic extract or each phenolic compound against 4-hydroxynonenal-induced carbonyl stress and toxicity.en_US
dc.language.isoenen_US
dc.publisherGeorg Thieme Verlagen_US
dc.relation.ispartofPlanta Medicaen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCardiomyocytesen_US
dc.subjectHydroxytyrosolen_US
dc.subjectOlea europaeaen_US
dc.subjectOlive leaf extractionen_US
dc.subjectQuercetinen_US
dc.titleOlive leaf extracts protect cardiomyocytes against 4-hydroxynonenal-induced toxicity in vitro: Comparison with oleuropein, hydroxytyrosol, and quercetinen_US
dc.typeArticleen_US
dc.departmentIzmir Institute of Technology. Chemical Engineeringen_US
dc.identifier.volume80en_US
dc.identifier.issue12en_US
dc.identifier.startpage984en_US
dc.identifier.endpage992en_US
dc.identifier.wosWOS:000340904300005
dc.identifier.scopusSCOPUS:2-s2.0-84906936735
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1055/s-0034-1382881-
dc.relation.doi10.1055/s-0034-1382881en_US
dc.coverage.doi10.1055/s-0034-1382881en_US
item.fulltextWith Fulltext-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeArticle-
item.languageiso639-1en-
item.cerifentitytypePublications-
item.grantfulltextopen-
Appears in Collections:Chemical Engineering / Kimya Mühendisliği
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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