Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/6504
Title: Organogenesis from transformed tomato explants
Authors: Frary, Anne
Van Eck, Joyce
Frary, Anne
Izmir Institute of Technology. Molecular Biology and Genetics
Keywords: Tomato
Genetic transformation
Lycopersicon esculentum
Transgenic plant
Organogenesis
Issue Date: 2005
Publisher: Humana Press
Source: Frary, A., and Van Eck, J. (2005). Organogenesis from transformed tomato explants. Methods in Molecular Biology, 286, 141-150.
Abstract: Tomato was one of the first crops for which a genetic transformation system was reported involving regeneration by organogenesis from Agrobacterium-transformed explants. Since the initial reports, various factors have been studied that affect the efficiency of tomato transformation and the technique has been useful for the isolation and identification of many genes involved in plant disease resistance, morphology and development. In this method, cotyledon explants from in vitro-grown seedlings are precultured overnight on a tobacco suspension feeder layer. The explants are then inoculated with Agrobacterium and returned to the feeder layer for a 2-d period of cocultivation. After cocultivation, the explants are transferred to an MS-based selective regeneration medium containing zeatin. Regenerated shoots are then rooted on a separate selective medium. This protocol has been used with several tomato cultivars and routinely yields transformation efficiencies of 10-15%.
URI: http://hdl.handle.net/11147/6504
ISSN: 1064-3745
1940-6029
1064-3745
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection

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