Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/6483
Title: Newly developed SSR markers reveal genetic diversity and geographical clustering in spinach (Spinacia oleracea)
Authors: Göl, Şurhan
Göktay, Mehmet
Allmer, Jens
Doğanlar, Sami
Frary, Anne
Keywords: Genetic diversity
Genomic SSRs
Microsatellites
Next generation sequencing
Population structure
Spinacia oleracea
Issue Date: Aug-2017
Publisher: Springer Verlag
Source: Göl, Ş., Göktay, M., Allmer, J., Doğanlar, S., and Frary, A (2017). Newly developed SSR markers reveal genetic diversity and geographical clustering in spinach (Spinacia oleracea). Molecular Genetics and Genomics, 292(4), 847-855. doi:10.1007/s00438-017-1314-4
Abstract: Spinach is a popular leafy green vegetable due to its nutritional composition. It contains high concentrations of vitamins A, E, C, and K, and folic acid. Development of genetic markers for spinach is important for diversity and breeding studies. In this work, Next Generation Sequencing (NGS) technology was used to develop genomic simple sequence repeat (SSR) markers. After cleaning and contig assembly, the sequence encompassed 2.5% of the 980 Mb spinach genome. The contigs were mined for SSRs. A total of 3852 SSRs were detected. Of these, 100 primer pairs were tested and 85% were found to yield clear, reproducible amplicons. These 85 markers were then applied to 48 spinach accessions from worldwide origins, resulting in 389 alleles with 89% polymorphism. The average gene diversity (GD) value of the markers (based on a GD calculation that ranges from 0 to 0.5) was 0.25. Our results demonstrated that the newly developed SSR markers are suitable for assessing genetic diversity and population structure of spinach germplasm. The markers also revealed clustering of the accessions based on geographical origin with clear separation of Far Eastern accessions which had the overall highest genetic diversity when compared with accessions from Persia, Turkey, Europe, and the USA. Thus, the SSR markers have good potential to provide valuable information for spinach breeding and germplasm management. Also they will be helpful for genome mapping and core collection establishment.
URI: http://doi.org/10.1007/s00438-017-1314-4
http://hdl.handle.net/11147/6483
ISSN: 1617-4615
1617-4623
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

Files in This Item:
File Description SizeFormat 
6483.pdfMakale754.69 kBAdobe PDFThumbnail
View/Open
Show full item record

CORE Recommender

SCOPUSTM   
Citations

10
checked on Sep 24, 2022

WEB OF SCIENCETM
Citations

15
checked on Sep 24, 2022

Page view(s)

92
checked on Sep 26, 2022

Download(s)

232
checked on Sep 26, 2022

Google ScholarTM

Check

Altmetric


Items in GCRIS Repository are protected by copyright, with all rights reserved, unless otherwise indicated.