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Title: Effects of intraperitoneal injection of allogeneic bone marrow-derived mesenchymal stem cells on bronchiolitis obliterans in mice model
Authors: Işık, Sakine
Uzuner, Nevin
Karaman, Meral
Karaman, Özkan
Kıray, Müge
Kozanoğlu, İlknur
Bağrıyanık, Hüsnü Alper
Arıkan Ayyıldız, Zeynep
Kartal Yandım, Melis
Baran, Yusuf
Keywords: Bronchiolitis obliterans
Intraperitoneal administration
Mesenchymal stem cells
Murine model
Issue Date: Jun-2017
Publisher: Tehran University of Medical Sciences
Source: Işık, S., Uzuner, N., Karaman, M., Karaman, Ö., Kıray, M., Kozanoğlu, İ., Bağrıyanık, H. A., Arıkan Ayyıldız, Z., Kartal Yandım, M., and Baran, Y. (2017). Effects of intraperitoneal injection of allogeneic bone marrow-derived mesenchymal stem cells on bronchiolitis obliterans in mice model. Iranian Journal of Allergy, Asthma and Immunology, 16(3), 205-218.
Abstract: Bone marrow-derived mesenchymal stem cells (BMSCs) can ameliorate a variety of lung diseases such as asthma, lung fibrosis, and acute lung injury by its anti-inflammatory and immunmodulatory effects. In this study, we developed a mouse model of bronchiolitis obliterans (BO) and evaluated the effects of the intraperitoneal administration of BMSCs on lung histopathology and cytokine levels. 25 BALB/c mice were divided into four groups; control group (Group I), BO developed and 1x106 BMSCs-injected group (Group II), non-BO, 1x106 BMSCs-injected group (Group III), and BO developed and saline-injected group (Group IV). Histological and immunohistochemical findings of the lung tissue and the migration of BMSCs to the lung were evaluated using light and confocal microscopy techniques. Confocal microscopy evaluations showed that there was no noteworthy amount of BMSCs in the lung tissue of group III while significant amount of BMSCs was detected in group II. Wall thicknesses of terminal bronchiole and periterminal bronchiolar collagen deposition were significantly lower in group II compared to the group IV (p<0.05). Furthermore, according to the immunohistochemical staining results, CD3, CD4, CD8, CD20, CD68 and neutrophil elastase positive immune cells of group II were stained more positive than group IV cells (p<0.05). IFN-ã IL-2 and TNF-á levels in bronchoalveolar lavage fluid (BALF) were significantly lower in group II compared to group IV (p<0.05). The findings of this study indicate that intraperitoneally administered BMSCs have potent effects on histopatological changes of the lung tissue and cytokine levels in the murine model of BO.
ISSN: 1735-1502
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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