Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/6423
Title: Effects of cell-mediated osteoprotegerin gene transfer and mesenchymal stem cell applications on orthodontically induced root resorption of rat teeth
Authors: Amuk, Nisa Gül
Kurt, Gökmen
Baran, Yusuf
Seyrantepe, Volkan
Kartal Yandım, Melis
Adan, Aysun
Akyıldız Demir, Seçil
Kiraz, Yağmur
Sönmez, Mehmet Fatih
Baran, Yusuf
Seyrantepe, Volkan
Kartal Yandım, Melis
Adan, Aysun
Akyıldız Demir, Seçil
Kiraz, Yağmur
Izmir Institute of Technology. Molecular Biology and Genetics
Keywords: Mesenchymal stem cells
Osteoprotegerin
Gene transfer
Orthodontic appliances
Osteoclastic cells
Teeth
Issue Date: Jun-2017
Publisher: Oxford University Press
Source: Amuk, N. G., Kurt, G., Baran, Y., Seyrantepe, V., Kartal Yandım, M., Adan, A., Akyıldız Demir, S., Kiraz, Y., and Sönmez, M. F. (2017). Effects of cell-mediated osteoprotegerin gene transfer and mesenchymal stem cell applications on orthodontically induced root resorption of rat teeth. European Journal of Orthodontics, 39(3), 235-242. doi:10.1093/ejo/cjw054
Abstract: Aim: The aim of this study is to evaluate and compare therapeutic effects of mesenchymal stem cell (MSCs) and osteoprotegerin (OPG) gene transfer applications on inhibition and/or repair of orthodontically induced inflammatory root resorption (OIIRR). Materials and methods: Thirty Wistar rats were divided into four groups as untreated group (negative control), treated with orthodontic appliance group (positive control), MSCs injection group, and OPG transfected MSCs [gene therapy (GT) group]. About 100 g of orthodontic force was applied to upper first molar teeth of rats for 14 days. MSCs and transfected MSC injections were performed at 1st, 6th, and 11th days to the MSC and GT group rats. At the end of experiment, upper first molar teeth were prepared for genetical, scanning electron microscopy (SEM), fluorescent microscopy, and haematoxylin eosin-tartrate resistant acid phosphatase staining histological analyses. Number of total cells, number of osteoclastic cells, number of resorption lacunae, resorption area ratio, SEM resorption ratio, OPG, RANKL, Cox-2 gene expression levels at the periodontal ligament (PDL) were calculated. Paired t-test, Kruskal-Wallis, and chi-square tests were performed. Results: Transferred MSCs showed marked fluorescence in PDL. The results revealed that number of osteoclastic cells, resorption lacunae, resorption area ratio, RANKL, and Cox-2 were reduced after single MSC injections significantly (P < 0.05). GT group showed the lowest number of osteoclastic cells (P < 0.01), number of resorption lacunae, resorption area ratio, and highest OPG expression (P < 0.001). Conclusions: Taken together all these results, MSCs and GT showed marked inhibition and/or repair effects on OIIRR during orthodontic treatment on rats.
URI: http://doi.org/10.1093/ejo/cjw054
http://hdl.handle.net/11147/6423
ISSN: 0141-5387
0141-5387
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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