Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/6337
Title: Flow cytometry: basic principles and applications
Authors: Adan, Aysun
Alizada, Günel
Kiraz, Yağmur
Baran, Yusuf
Nalbant, Ayten
Alizada, Günel
Baran, Yusuf
Nalbant, Ayten
Izmir Institute of Technology. Molecular Biology and Genetics
Keywords: Apoptosis
Flow cytometer
Fluorescence
Light scattering
Immunophenotyping
Issue Date: Feb-2017
Publisher: Taylor and Francis Ltd.
Source: Adan, A., Alizada, G., Kiraz, Y., Baran, Y., and Nalbant, A.(2017). Flow cytometry: basic principles and applications. Critical Reviews in Biotechnology, 37(2), 163-176. doi:10.3109/07388551.2015.1128876
Abstract: Flow cytometry is a sophisticated instrument measuring multiple physical characteristics of a single cell such as size and granularity simultaneously as the cell flows in suspension through a measuring device. Its working depends on the light scattering features of the cells under investigation, which may be derived from dyes or monoclonal antibodies targeting either extracellular molecules located on the surface or intracellular molecules inside the cell. This approach makes flow cytometry a powerful tool for detailed analysis of complex populations in a short period of time. This review covers the general principles and selected applications of flow cytometry such as immunophenotyping of peripheral blood cells, analysis of apoptosis and detection of cytokines. Additionally, this report provides a basic understanding of flow cytometry technology essential for all users as well as the methods used to analyze and interpret the data. Moreover, recent progresses in flow cytometry have been discussed in order to give an opinion about the future importance of this technology.
URI: http://doi.org/10.3109/07388551.2015.1128876
http://hdl.handle.net/11147/6337
ISSN: 0738-8551
0738-8551
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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