Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/6137
Title: Barcode DNA length polymorphisms vs fatty acid profiling for adulteration detection in olive oil
Authors: Uncu, Ali Tevfik
Uncu, Ayşe Özgür
Frary, Anne
Doğanlar, Sami
Keywords: Capillary electrophoresis
Food authenticity
Food genomics
Traceability
Olive oil
Issue Date: Apr-2017
Publisher: Elsevier Ltd.
Source: Uncu, A. T., Uncu, A. Ö., Frary, A., and Doğanlar, S. (2017). Barcode DNA length polymorphisms vs fatty acid profiling for adulteration detection in olive oil. Food Chemistry, 221, 1026-1033. doi:10.1016/j.foodchem.2016.11.059
Abstract: The aim of this study was to compare the performance of a DNA-barcode assay with fatty acid profile analysis to authenticate the botanical origin of olive oil. To achieve this aim, we performed a PCR-capillary electrophoresis (PCR-CE) approach on olive oil: seed oil blends using the plastid . trnL (UAA) intron barcode. In parallel to genomic analysis, we subjected the samples to gas chromatography analysis of fatty acid composition. While the PCR-CE assay proved equally efficient as gas chromatography analysis in detecting adulteration with soybean, palm, rapeseed, sunflower, sesame, cottonseed and peanut oils, it was superior to the widely utilized analytical chemistry approach in revealing the adulterant species and detecting small quantities of corn and safflower oils in olive oil. Moreover, the DNA-based test correctly identified all tested olive oil: hazelnut oil blends whereas it was not feasible to detect hazelnut oil adulteration through fatty acid profile analysis. Thus, the present research has shown the feasibility of a PCR-CE barcode assay to detect adulteration in olive oil.
URI: http://doi.org/10.1016/j.foodchem.2016.11.059
http://hdl.handle.net/11147/6137
ISSN: 0308-8146
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

Files in This Item:
File Description SizeFormat 
6137.pdfMakale915.6 kBAdobe PDFThumbnail
View/Open
Show full item record

CORE Recommender

SCOPUSTM   
Citations

26
checked on Nov 27, 2021

WEB OF SCIENCETM
Citations

29
checked on Nov 27, 2021

Page view(s)

44
checked on Dec 3, 2021

Download(s)

70
checked on Dec 3, 2021

Google ScholarTM

Check

Altmetric


Items in GCRIS Repository are protected by copyright, with all rights reserved, unless otherwise indicated.