Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/6103
Title: Tailoring conformation-induced chromism of polythiophene copolymers for nucleic acid assay at resource limited settings
Authors: Rajwar, Deepa
Ammanath, Gopal
Cheema, Jamal Ahmed
Palaniappan, Alagappan
Yıldız, Ümit Hakan
Liedberg, Bo
Keywords: miRNA
PNA
Colorimetric assay
Conjugated polyelectrolytes
Polythiophene
Issue Date: 20-Apr-2016
Publisher: American Chemical Society
Source: Rajwar, D., Ammanath, G., Cheema, J. A., Palaniappan, A., Yıldız, Ü. H., and Liedberg, B. (2016). Tailoring conformation-induced chromism of polythiophene copolymers for nucleic acid assay at resource limited settings. ACS Applied Materials and Interfaces, 8(13), 8349-8357. doi:10.1021/acsami.5b12171
Abstract: Here we report on the design and synthesis of cationic water-soluble thiophene copolymers as reporters for colorimetric detection of microRNA (miRNA) in human plasma. Poly(3-alkoxythiophene) (PT) polyelectrolytes with controlled ratios of pendant groups such as triethylamine/1-methyl imidazole were synthesized for optimizing interaction with target miRNA sequence (Tseq). Incorporation of specific peptide nucleic acid (PNA) sequences with the cationic polythiophenes yielded distinguishable responses upon formation of fluorescent PT-PNA-Tseq triplex and weakly fluorescent PT-Tseq duplex, thereby enabling selective detection of target miRNA. Unlike homopolymers of PT (hPT), experimental results indicate the possibility of utilizing copolymers of PT (cPT) with appropriate ratios of pendant groups for miRNA assay in complex matrices such as plasma. As an illustration, colorimetric responses were obtained for lung cancer associated miRNA sequence (mir21) in human plasma, with a detection limit of 10 nM, illustrating the feasibility of proposed methodology for clinical applications without involving sophisticated instrumentation. The described methodology therefore possesses high potential for low-cost nucleic acid assays in resource-limited settings.
URI: http://doi.org/10.1021/acsami.5b12171
http://hdl.handle.net/11147/6103
ISSN: 1944-8244
1944-8252
Appears in Collections:Chemistry / Kimya
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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