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dc.contributor.authorNalbant, Ayten
dc.contributor.authorKant, Melis
dc.date.accessioned2017-07-26T11:55:54Z
dc.date.available2017-07-26T11:55:54Z
dc.date.issued2016-10
dc.identifier.citationNalbant, A., and Kant, M. (2016). Bacterial heat shock protein GroEL (Hsp64) exerts immunoregulatory effects on T cells by utilizing apoptosis. PLoS ONE, 11(10). doi:10.1371/journal.pone.0164380en_US
dc.identifier.issn1932-6203
dc.identifier.urihttp://doi.org/10.1371/journal.pone.0164380
dc.identifier.urihttp://hdl.handle.net/11147/6028
dc.description.abstractAggregatibacter actinomycetemcomitans (Aa) expresses a 64-kDa GroEL protein belonging to the heat shock family of proteins. This protein has been shown to influence human host cells, but the apoptotic capacity of the GroEL protein regarding T cells is not yet known. The purpose of this study was to investigate the ability of A. actinomycetemcomitans GroEL (AaGroEL) protein to induce human peripheral blood T-cell apoptosis. Endogenous, purified AaGroEL protein was used as an antigen. In AaGroEL-treated T cells, the data indicated that phosphatidylserine exposure, an early apoptotic event, was dose- and time-dependent. The AaGroEL-treated T cells were also positive for active caspase-3 in a dose-dependent manner. The rate of AaGroEL-induced apoptosis was suppressed by the addition of the general caspase inhibitor Z-VAD-FMK. Furthermore, cleaved caspase-8 bands (40/36 kDa and 23 kDa) were identified in cells responding to AaGroEL. DNA fragmentation was also detected in the AaGroEL-treated T cells. Overall, we demonstrated that the endogenous GroEL from A. actinomycetemcomitans has the capacity to induce T-cell apoptosis. © 2016 Nalbant, Kant. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (106T417)en_US
dc.language.isoengen_US
dc.publisherPublic Library of Scienceen_US
dc.relationinfo:eu-repo/grantAgreement/TUBITAK/TBAG/106T417en_US
dc.relation.isversionof10.1371/journal.pone.0164380en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBacterial proteinen_US
dc.subjectCaspase inhibitoren_US
dc.subjectApoptosisen_US
dc.subjectMembrane proteinsen_US
dc.subjectDNA fragmentationen_US
dc.subjectT cellsen_US
dc.subjectAggregatibacter actinomycetemcomitansen_US
dc.titleBacterial heat shock protein GroEL (Hsp64) exerts immunoregulatory effects on T cells by utilizing apoptosisen_US
dc.typearticleen_US
dc.contributor.authorIDTR115306en_US
dc.contributor.iztechauthorNalbant, Ayten
dc.contributor.iztechauthorKant, Melis
dc.relation.journalPLoS ONEen_US
dc.contributor.departmentİYTE, Fen Fakültesi, Moleküler Biyoloji ve Genetik Bölümüen_US
dc.identifier.volume11en_US
dc.identifier.issue10en_US
dc.identifier.wosWOS:000385505800062
dc.identifier.scopusSCOPUS.2-s2.0-84991380543
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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