Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/5952
Title: Utilization of orange peel, a food industrial waste, in the production of exo-polygalacturonase by pellet forming Aspergillus sojae
Authors: Büyükkileci, Ali Oğuz
Lahore, Marcelo Fernandez
Tarı, Canan
Büyükkileci, Ali Oğuz
Tarı, Canan
Izmir Institute of Technology. Food Engineering
Keywords: Aspergillus sojae
Exo-polygalacturonase
Food industry waste
Low-cost medium
Orange peel
Pelletizing
Issue Date: Mar-2015
Publisher: Springer Verlag
Source: Büyükkileci, A. O., Lahore, M. F., and Tarı, C. (2015). Utilization of orange peel, a food industrial waste, in the production of exo-polygalacturonase by pellet forming Aspergillus sojae. Bioprocess and Biosystems Engineering, 38(4), 749-760. doi:10.1007/s00449-014-1315-1
Abstract: The production of exo-polygalacturonase (exo-PG) from orange peel (OP), a food industrial waste, using Aspergillus sojae was studied in submerged culture. A simple, low-cost, industrially significant medium formulation, composed of only OP and (NH4)2SO4 (AS) was developed. At an inoculum size of 2.8 × 103 spores/mL, growth was in the form of pellets, which provided better mixing of the culture broth and higher exo-PG activity. These pellets were successfully used as an inoculum for bioreactors and 173.0 U/mL exo-PG was produced. Fed-batch cultivation further enhanced the exo-PG activity to 244.0 U/mL in 127.5 h. The final morphology in the form of pellets is significant to industrial fermentation easing the subsequent downstream processing. Furthermore, the low pH trend obtained during this fermentation serves an advantage to fungal fermentations prone to contamination problems. As a result, an economical exo-PG production process was defined utilizing a food industrial by-product and producing high amount of enzyme.
URI: https://doi.org/10.1007/s00449-014-1315-1
http://hdl.handle.net/11147/5952
ISSN: 1615-7591
1615-7591
Appears in Collections:Food Engineering / Gıda Mühendisliği
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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