Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/5941
Title: One step forward, two steps back; Xeno-MicroRNAs reported in breast milk are artifacts
Authors: Bağcı, Caner
Allmer, Jens
Bağcı, Caner
Allmer, Jens
Izmir Institute of Technology. Molecular Biology and Genetics
Keywords: Messenger RNA
MicroRNAs
Breast milk
Arabidopsis thaliana
Gene expression regulation
Nicotiana tabacum
Issue Date: Jan-2016
Publisher: Public Library of Science
Source: Bağcı, C., and Allmer, J. (2016). One step forward, two steps back; Xeno-MicroRNAs reported in breast milk are artifacts. PLoS ONE, 11(1). doi:10.1371/journal.pone.0145065
Abstract: Background: MicroRNAs (miRNAs) are short RNA sequences that guide post-transcriptional regulation of gene expression via complementarity to their target mRNAs. Discovered only recently, miRNAs have drawn a lot of attention. Multiple protein complexes interact to first cleave a hairpin from nascent RNA, export it into the cytosol, trim its loop, and incorporate it into the RISC complex which is important for binding its target mRNA. This process works within one cell, but circulating miRNAs have been described suggesting a role in cell-cell communication. Motivation: Viruses and intracellular parasites like Toxoplasma gondii use miRNAs to manipulate host gene expression from within the cellular environment. However, recent research has claimed that a rice miRNA may regulate human gene expression. Despite ongoing debates about these findings and general reluctance to accept them, a recent report claimed that foodborne plant miRNAs pass through the digestive tract, travel through blood to be incorporated by alveolar cells excreting milk. The miRNAs are then said to have some immunerelated function in the newborn. Principal Findings: We acquired the data that supports their claim and performed further analyses. In addition to the reported miRNAs, we were able to detect almost complete mRNAs and found that the foreign RNA expression profiles among samples are exceedingly similar. Inspecting the source of the data helped understand how RNAs could contaminate the samples. Conclusion: Viewing these findings in context with the difficulties foreign RNAs face on their route into breast milk and the fact that many identified foodborne miRNAs are not from actual food sources, we can conclude beyond reasonable doubt that the original claims and evidence presented may be due to artifacts. We report that the study claiming their existence is more likely to have detected RNA contamination than miRNAs.
URI: http://doi.org/10.1371/journal.pone.0145065
http://hdl.handle.net/11147/5941
ISSN: 1932-6203
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Sürdürülebilir Yeşil Kampüs Koleksiyonu / Sustainable Green Campus Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

Files in This Item:
File Description SizeFormat 
5941.PDFMakale695.57 kBAdobe PDFThumbnail
View/Open
Show full item record

CORE Recommender

SCOPUSTM   
Citations

23
checked on Sep 18, 2021

WEB OF SCIENCETM
Citations

17
checked on Sep 18, 2021

Page view(s)

12
checked on Sep 19, 2021

Download(s)

14
checked on Sep 19, 2021

Google ScholarTM

Check

Altmetric


Items in GCRIS Repository are protected by copyright, with all rights reserved, unless otherwise indicated.