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dc.contributor.authorÇinçin, Zeynep Birsu
dc.contributor.authorÜnlü, Miray
dc.contributor.authorKıran, Bayram
dc.contributor.authorBireller, Elif Sinem
dc.contributor.authorBaran, Yusuf
dc.contributor.authorÇakmakoğlu, Bedia
dc.date.accessioned2017-07-10T08:49:36Z
dc.date.available2017-07-10T08:49:36Z
dc.date.issued2015-06-29
dc.identifier.citationÇinçin, Z. B., Ünlü, M., Kıran, B., Bireller, E. S., Baran, Y., and Çakmakoğlu, B. (2015). Anti-proliferative, apoptotic and signal transduction effects of hesperidin in non-small cell lung cancer cells. Cellular Oncology, 38(3), 195-204. doi:10.1007/s13402-015-0222-zen_US
dc.identifier.issn2211-3428
dc.identifier.urihttps://doi.org/10.1007/s13402-015-0222-z
dc.identifier.urihttp://hdl.handle.net/11147/5903
dc.description.abstractPurpose: Hesperidin, a glycoside flavonoid, is thought to act as an anti-cancer agent, since it has been found to exhibit both pro-apoptotic and anti-proliferative effects in several cancer cell types. The mechanisms underlying hesperidin-induced growth arrest and apoptosis are, however, not well understood. Here, we aimed to investigate the anti-proliferative and apoptotic effects of hesperidin on non-small cell lung cancer (NSCLC) cells and to investigate the mechanisms involved. Methods: The anti-proliferative and apoptotic effects of hesperidin on two NSCLC-derived cell lines, A549 and NCI-H358, were determined using a WST-1 colorimetric assay, a LDH cytotoxicity assay, a Cell Death Detection assay, an AnnexinV-FITC assay, a caspase-3 assay and a JC-1 assay, respectively, all in a time- and dose-dependent manner. As a control, non-cancerous MRC-5 lung fibroblasts were included. Changes in whole genome gene expression profiles were assessed using an Illumina Human HT-12v4 beadchip microarray platform, and subsequent data analyses were performed using an Illumina Genome Studio and Ingenuity Pathway Analyser (IPA). Results: We found that after hesperidin treatment, A549 and NCI-H358 cells exhibited decreasing cell proliferation and increasing caspase-3 and other apoptosis-related activities, in conjunction with decreasing mitochondrial membrane potential activities, in a dose- and time-dependent manner. Through a GO analysis, by which changes in gene expression profiles were compared, we found that the FGF and NF-κB signal transduction pathways were most significantly affected in the hesperidin treated NCI-H358 and A549 NSCLC cells. Conclusions: Our results indicate that hesperidin elicits an in vitro growth inhibitory effect on NSCLC cells by modulating immune response-related pathways that affect apoptosis. When confirmed in vivo, hesperidin may serve as a novel anti-proliferative agent for non-small cell lung cancer.en_US
dc.description.sponsorshipIstanbul University Scientific Research Project (9205)en_US
dc.language.isoengen_US
dc.publisherSpringeren_US
dc.relation.isversionof10.1007/s13402-015-0222-zen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAnti-proliferative effecten_US
dc.subjectApoptosisen_US
dc.subjectGene expression profileen_US
dc.subjectHesperidinen_US
dc.subjectNon-small cell lung canceren_US
dc.titleAnti-proliferative, apoptotic and signal transduction effects of hesperidin in non-small cell lung cancer cellsen_US
dc.typearticleen_US
dc.contributor.authorIDTR119193en_US
dc.contributor.institutionauthorÜnlü, Miray
dc.contributor.institutionauthorBaran, Yusuf
dc.relation.journalCellular Oncologyen_US
dc.contributor.departmentİYTE, Fen Fakültesi, Moleküler Biyoloji ve Genetik Bölümüen_US
dc.identifier.volume38en_US
dc.identifier.issue3en_US
dc.identifier.startpage195en_US
dc.identifier.endpage204en_US
dc.identifier.wosWOS:000355189100003
dc.identifier.scopusSCOPUS:2-s2.0-84929951881
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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