Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/5871
Title: Differentiation of normal and cancer cell adhesion on custom designed protein nanopatterns
Authors: Horzum, Utku
Özdil, Berrin
Pesen Okvur, Devrim
Horzum, Utku
Özdil, Berrin
Pesen Okvur, Devrim
Izmir Institute of Technology. Molecular Biology and Genetics
Keywords: Electron beam lithography
Cancer
Fibronectin
Focal adhesion
Laminin
Nanopattern
Issue Date: 12-Aug-2015
Publisher: American Chemical Society
Source: Horzum, U., Özdil, B., and Pesen Okvur, D. (2015). Differentiation of normal and cancer cell adhesion on custom designed protein nanopatterns. Nano Letters, 15(8), 5393-5406. doi:10.1021/acs.nanolett.5b01785
Abstract: Cell adhesion to the extracellular matrix is deregulated in metastasis. However, traditional surfaces used to study cell adhesion do not faithfully mimic the in vivo microenvironment. Electron beam lithography (EBL) is able to generate customized protein nanopatterns. Here, we used an EBL-based green lithography approach to fabricate homogeneous and gradient, single (fibronectin, K-casein) and double (fibronectin, laminin) active component protein nanopatterns with micrometer scale spacing to investigate differences in adhesion of breast cancer cells (BCC) and normal mammary epithelial cells (NMEC). Our results showed that as expected, in contrast to NMEC, BCC were plastic: they tolerated nonadhesion promoting regions, adapted to flow and exploited gradients better. In addition, the number of focal adhesions but not their area appeared to be the dominant parameter for regulation of cell adhesion. Our findings also demonstrated that custom designed protein nanopatterns, which can properly mimic the in vivo microenvironment, enable realistic distinction of normal and cancerous cell adhesion.
URI: https://doi.org/10.1021/acs.nanolett.5b01785
http://hdl.handle.net/11147/5871
ISSN: 1530-6992
1530-6984
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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