Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/5826
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dc.contributor.authorAdan, Aysun-
dc.contributor.authorBaran, Yusuf-
dc.date.accessioned2017-06-30T13:10:13Z-
dc.date.available2017-06-30T13:10:13Z-
dc.date.issued2015-11-
dc.identifier.citationAdan, A., and Baran, Y. (2015). The pleiotropic effects of fisetin and hesperetin on human acute promyelocytic leukemia cells are mediated through apoptosis, cell cycle arrest, and alterations in signaling networks. Tumor Biology, 36(11), 8973-8984. doi:10.1007/s13277-015-3597-6en_US
dc.identifier.issn1423-0380-
dc.identifier.issn1010-4283-
dc.identifier.urihttps://doi.org/10.1007/s13277-015-3597-6-
dc.identifier.urihttp://hdl.handle.net/11147/5826-
dc.description.abstractFisetin and hesperetin, flavonoids from various plants, have several pharmaceutical activities including antioxidative, anti-inflammatory, and anticancer effects. However, studies elucidating the role and the mechanism(s) of action of fisetin and hesperetin in acute promyelocytic leukemia are absent. In this study, we investigated the mechanism of the antiproliferative and apoptotic actions exerted by fisetin and hesperetin on human HL60 acute promyelocytic leukemia cells. The viability of HL60 cells was evaluated using the MTT assay, apoptosis by annexin V/propidium iodide (PI) staining and cell cycle distribution using flow cytometry, and changes in caspase-3 enzyme activity and mitochondrial transmembrane potential. Moreover, we performed whole-genome microarray gene expression analysis to reveal genes affected by fisetin and hesperetin that can be important for developing of future targeted therapy. Based on data obtained from microarray analysis, we also described biological networks modulated after fisetin and hesperetin treatment by KEGG and IPA analysis. Fisetin and hesperetin treatment showed a concentration- and time-dependent inhibition of proliferation and induced G2/M arrest for both agents and G0/G1 arrest for hesperetin at only the highest concentrations. There was a disruption of mitochondrial membrane potential together with increased caspase-3 activity. Furthermore, fisetin- and hesperetin-triggered apoptosis was confirmed by annexin V/PI analysis. The microarray gene profiling analysis revealed some important biological pathways including mitogen-activated protein kinases (MAPK) and inhibitor of DNA binding (ID) signaling pathways altered by fisetin and hesperetin treatment as well as gave a list of genes modulated ≥2-fold involved in cell proliferation, cell division, and apoptosis. Altogether, data suggested that fisetin and hesperetin have anticancer properties and deserve further investigation.en_US
dc.language.isoenen_US
dc.publisherSAGE Publications Inc.en_US
dc.relation.ispartofTumor Biologyen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAcute promyelocytic leukemiaen_US
dc.subjectApoptosisen_US
dc.subjectFisetinen_US
dc.subjectGene profilingen_US
dc.subjectHesperetinen_US
dc.titleThe pleiotropic effects of fisetin and hesperetin on human acute promyelocytic leukemia cells are mediated through apoptosis, cell cycle arrest, and alterations in signaling networksen_US
dc.typeArticleen_US
dc.authoridTR119193en_US
dc.institutionauthorAdan, Aysun-
dc.institutionauthorBaran, Yusuf-
dc.departmentİzmir Institute of Technology. Molecular Biology and Geneticsen_US
dc.identifier.volume36en_US
dc.identifier.issue11en_US
dc.identifier.startpage8973en_US
dc.identifier.endpage8984en_US
dc.identifier.wosWOS:000366143100087en_US
dc.identifier.scopus2-s2.0-84949109653en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1007/s13277-015-3597-6-
dc.identifier.pmid26081618en_US
dc.relation.doi10.1007/s13277-015-3597-6en_US
dc.coverage.doi10.1007/s13277-015-3597-6en_US
dc.identifier.wosqualityQ2-
dc.identifier.scopusqualityQ1-
item.grantfulltextopen-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.openairetypeArticle-
item.languageiso639-1en-
item.fulltextWith Fulltext-
crisitem.author.dept04.03. Department of Molecular Biology and Genetics-
crisitem.author.dept04.03. Department of Molecular Biology and Genetics-
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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