Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/5758
Title: Investigation of the structure of alpha-lactalbumin protein nanotubes using optical spectroscopy
Authors: Tarhan, Özgür
Tarhan, Enver
Harsa, Şebnem
Tarhan, Özgür
Tarhan, Enver
Harsa, Şebnem
Keywords: FTIR
Microscopy
Protein nanotubes
Raman
α-lactalbumin
Bacillus licheniformis
Issue Date: Feb-2014
Publisher: Cambridge University Press
Source: Tarhan, O., Tarhan, E., and Harsa, Ş. (2014). Investigation of the structure of alpha-lactalbumin protein nanotubes using optical spectroscopy. Journal of Dairy Research, 81(1), 98-106. doi:10.1017/S0022029913000629
Abstract: Alpha-lactalbumin (α-la) is one of the major proteins in whey. When partially hydrolysed with Bacillus licheniformis protease, it produces nanotubular structures in the presence of calcium ions by a self-assembly process. This study presents investigation of α-la protein structure during hydrolysis and nanotube formation using optical spectroscopy. Before spectroscopic measurements, nanotubes were examined with microscopy. The observed α-la nanotubes (α-LaNTs) were in the form of regular hollo strands with a diameter of about 20 nm and the average length of 1 μm. Amide and backbone vibration bands of the Raman spectra displayed remarkable conformational changes in α and β domains in the protein structure during nanotube growth. This was confirmed by the Fourier-transform infrared (FTIR) spectroscopy data. Also, FTIR analysis revealed certain bands at calcium (Ca++) binding sites of COO- groups in hydrolysed protein. These sites might be critical in nanotube elongation.
URI: https://doi.org/10.1017/S0022029913000629
http://hdl.handle.net/11147/5758
ISSN: 0022-0299
0022-0299
Appears in Collections:Food Engineering / Gıda Mühendisliği
Physics / Fizik
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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