Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/5640
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dc.contributor.authorÇinçin, Zeynep Birsu-
dc.contributor.authorÜnlü, Miray-
dc.contributor.authorKıran, Bayram-
dc.contributor.authorBireller, Elif Sinem-
dc.contributor.authorBaran, Yusuf-
dc.contributor.authorÇakmakoğlu, Bedia-
dc.date.accessioned2017-05-30T07:49:17Z
dc.date.available2017-05-30T07:49:17Z
dc.date.issued2014-08
dc.identifier.citationÇinçin, Z.B., Ünlü, M., Kıran, B., Bireller, E.S., Baran, Y., and Çakmakoğlu, B. (2014). Molecular mechanisms of quercitrin-induced apoptosis in non-small cell lung cancer. Archives of Medical Research, 45(6), 445-454. doi:10.1016/j.arcmed.2014.08.002en_US
dc.identifier.issn0188-4409
dc.identifier.issn0188-4409-
dc.identifier.issn1873-5487-
dc.identifier.urihttps://doi.org/10.1016/j.arcmed.2014.08.002
dc.identifier.urihttp://hdl.handle.net/11147/5640
dc.description.abstractBackground and Aims: Quercitrin (QR; quercetin-3-O-rhamnoside) has been used previously as an antibacterial agent and has been shown to inhibit the oxidation of low-density lipoproteins and prevent an allergic reaction. Furthermore, it was demonstrated that quercitrin exerts protective effects against H2O2-induced dysfunction in lung fibroblast cells. However, the mechanisms of quercitrin effects on cancer cell proliferation and apoptosis is not well understood. The aim of this study is to investigate the cytotoxic and apoptotic effects of quercitrin and the molecular mechanisms of quercitrin-induced apoptosis in non-small cell lung cancer (NSCLC) cell lines. Methods: Time- and dose-dependent antiproliferative and apoptotic effects of quercitrin determined by WST-1cell proliferation assay, lactate dehydrogenase (LDH) cytotoxicity assay, determination of nucleosome enrichment factor, changes in caspase-3 activity, loss of mitochondrial membrane potential (MMP) and also the localization of phosphatidylserine in the plasma membrane. Changes in whole genome gene expression levels were examined by Illumina Human HT-12v4 beadchip microarrays. Results: There were significant increases in caspase-3 activity, loss of MMP, and increases in apoptotic cell population in response to quercitrin in A549 and NCI-H358 NSCLC cells in a time- and dose-dependent manner. Conclusion: Our results demonstrated that genes involved in leukocyte transendothelial migration, cell adhesion and phosphatidylinositol signaling system pathways were the most statistically significant pathways in NCI-H358 and A549cells. These results revealed that quercitrin has antiproliferative and apoptotic effects on lung cancer cells by modulating the immune response. After confirming its anticarcinogenic effects invivo, quercitrin could be a novel and strong anticancer agent against NSCLC.en_US
dc.description.sponsorshipIstanbul University (9205)en_US
dc.language.isoenen_US
dc.publisherElsevier Ltd.en_US
dc.relation.ispartofArchives of Medical Researchen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectApoptosisen_US
dc.subjectMicroarrayen_US
dc.subjectNon-small cell lung canceren_US
dc.subjectQuercitrinen_US
dc.subjectCancer cellsen_US
dc.titleMolecular mechanisms of quercitrin-induced apoptosis in non-small cell lung canceren_US
dc.typeArticleen_US
dc.authoridTR119193en_US
dc.institutionauthorBaran, Yusuf-
dc.departmentIzmir Institute of Technology. Molecular Biology and Geneticsen_US
dc.identifier.volume45en_US
dc.identifier.issue6en_US
dc.identifier.startpage445en_US
dc.identifier.endpage454en_US
dc.identifier.wosWOS:000342548200001en_US
dc.identifier.scopus2-s2.0-84908073606en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1016/j.arcmed.2014.08.002-
dc.identifier.pmid25193878en_US
dc.relation.doi10.1016/j.arcmed.2014.08.002en_US
dc.coverage.doi10.1016/j.arcmed.2014.08.002en_US
item.fulltextWith Fulltext-
item.cerifentitytypePublications-
item.languageiso639-1en-
item.openairetypeArticle-
item.grantfulltextopen-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
crisitem.author.dept04.03. Department of Molecular Biology and Genetics-
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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