Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/5590
Title: Fabrication of 3D controlled in vitro microenvironments
Authors: Özdil, Berrin
Önal, Sevgi
Oruç, Tuğçe
Pesen Okvur, Devrim
Özdil, Berrin
Önal, Sevgi
Oruç, Tuğçe
Pesen Okvur, Devrim
Izmir Institute of Technology. Molecular Biology and Genetics
Keywords: Hydrogel
Lab-on-a-chip
Matrigel
Microenvironment
Microfluidic device
Polydimethylsiloxane
Issue Date: 2014
Publisher: Elsevier Ltd.
Source: Özdil, B., Önal, S., Oruç, T., and Pesen Okvur, D. (2014). Fabrication of 3D Controlled in vitro Microenvironments. MethodsX, 1(1), 60-66. doi:10.1016/j.mex.2014.06.003
Abstract: Microfluidics-based lab-on-a-chips have many advantages, one of which is to provide physiologically relevant settings for cell biology experiments. Thus there is an ever increasing interest in their fabrication. Our goal is to construct three dimensional (3D) Controlled in vitro Microenvironments (CivMs) that mimic the in vivo microenvironments. Here, we present our optimized fabrication method that works for various lab-on-a-chip designs with a wide range of dimensions. The most crucial points are:While using one type of SU-8 photoresist (SU-2075), fine tuning of ramp, dwell time, spin speed, durations of soft bake, UV exposure and development allows fabrication of SU-8 masters with various heights from 40 to 600 μm.Molding PDMS (polydimethylsiloxane) at room temperature for at least two days instead of baking at higher temperatures prevents not only tears and bubbles in PDMS stamps but also cracks in the SU-8 master.3D nature of the CivMs is ensured by keeping the devices inverted during gel polymerization.
URI: https://doi.org/10.1016/j.mex.2014.06.003
http://hdl.handle.net/11147/5590
ISSN: 2215-0161
2215-0161
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection

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