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Title: Gossypol interferes with both type I and Type II topoisomerase activities without generating strand breaks
Authors: Şenarısoy, Müge
Cantürk, Pakize
Zencir, Sevil
Baran, Yusuf
Topçu, Zeki
Keywords: Anticancer drug research
DNA strand breaks
DNA topoisomerases
Gyrase inhibitor
Publisher: Humana Press
Source: Şenarısoy, M., Cantürk, P., Zencir, S., Baran, Y., and Topçu, Z. (2013). Gossypol interferes with both type I and Type II topoisomerase activities without generating strand breaks. Cell Biochemistry and Biophysics, 66(1), 199-204. doi:10.1007/s12013-012-9468-5
Abstract: A considerable number of agents with chemotherapeutic potentials reported over the past years were shown to interfere with the reactions of DNA topoisomerases, the essential enzymes that regulate conformational changes in DNA topology. Gossypol, a naturally occurring bioactive phytochemical is a chemopreventive agent against various types of cancer cell growth with a reported activity on mammalian topoisomerase II. The compounds targeting topoisomerases vary in their mode of action; class I compounds act by stabilizing covalent topoisomerase-DNA complexes resulting in DNA strand breaks while class II compounds interfere with the catalytic function of topoisomerases without generating strand breaks. In this study, we report Gossypol as the interfering agent with type I topoisomerases as well. We also carried out an extensive set of assays to analyze the type of interference manifested by Gossypol on DNA topoisomerases. Our results strongly suggest that Gossypol is a potential class II inhibitor as it blocked DNA topoisomerase reactions with no consequently formed strand breaks.
ISSN: 1085-9195
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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